Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 40% inhibition | Streptococcus thermophilus | |
iodoacetate | 5 mM, 64% inhibition | Streptococcus thermophilus | |
phenylmethylsulfonyl fluoride | 5 mM, complete inhibition | Streptococcus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
Gly-Pro-4-nitroanilide | pH 7.0, 50°C | Streptococcus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Streptococcus thermophilus | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
2 * 80000, SDS-PAGE | Streptococcus thermophilus |
160000 | - |
gel filtration | Streptococcus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus thermophilus | - |
ACA-DC 4 | - |
Streptococcus thermophilus ACA-DC 4 | - |
ACA-DC 4 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
285 | - |
- |
Streptococcus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro-Gly + H2O | 72% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Ala-Pro + Gly | - |
? | |
Arg-Pro 4-nitroanilide + H2O | 55% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Arg-Pro + 4-nitroaniline | - |
? | |
Gly-Pro 4-nitroanilide + H2O | - |
Streptococcus thermophilus | Gly-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-Arg-Pro + H2O | as active as Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Gly-Pro + Arg-Pro | - |
? | |
Ile-Pro-Ile + H2O | 34% of the activity with Gly-Pro-4-nitroanilide | Streptococcus thermophilus | Ile-Pro + Ile | - |
? | |
Val-Pro-Leu + H2O | 83% of the activity with Gly-Pro-nitroanilide | Streptococcus thermophilus | Val-Pro + Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 80000, SDS-PAGE | Streptococcus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
hydrolysis of Gly-Pro-4-nitroanilide | Streptococcus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | 40°C: about 15% of maximal activity, 60°C: about 40% of maximal activity | Streptococcus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 min in absence of substrate, 96% loss of activity | Streptococcus thermophilus |
70 | - |
1 min, complete loss of activity | Streptococcus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
hydrolysis of Gly-Pro-4-nitroanilide | Streptococcus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 90% of maximal activity, pH 9.0: about 85% of maximal activity, hydrolysis of Gly-Pro-4-nitroanilide | Streptococcus thermophilus |