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Literature summary for 3.4.14.11 extracted from

  • Khalid, N.M.; Marth, E.H.
    Purification and partial characterization of a prolyl-dipeptidyl aminopeptidase from Lactobacillus helveticus CNRZ 32 (1990), Appl. Environ. Microbiol., 56, 381-388.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Lactobacillus helveticus
Cu2+
-
Lactobacillus helveticus
diisopropylfluorophosphate
-
Lactobacillus helveticus
EDTA
-
Lactobacillus helveticus
Hg2+
-
Lactobacillus helveticus
iodoacetate
-
Lactobacillus helveticus
additional information not: DTT, 1.0-19 mM Lactobacillus helveticus
NEM
-
Lactobacillus helveticus
PCMB
-
Lactobacillus helveticus
phenylmethylsulfonyl fluoride
-
Lactobacillus helveticus
Zn2+
-
Lactobacillus helveticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1-2.5 mM, 40°C, 15 min, activation Lactobacillus helveticus
K+ 10-50 mM, 40°C, 15 min, activation Lactobacillus helveticus
Mg2+ 2.5 mM, 40°C, 15 min, activation Lactobacillus helveticus
Na+ 10-50 mM, 40°C, 15 min, activation Lactobacillus helveticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
x * 72000, native PAGE and SDS-PAGE Lactobacillus helveticus

Organism

Organism UniProt Comment Textmining
Lactobacillus helveticus
-
CNRZ 32
-
Lactobacillus helveticus CNRZ 32
-
CNRZ 32
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala 4-nitroanilide + H2O 2% of the activity with Gly-Pro 4-nitroanilide Lactobacillus helveticus Ala-Ala + 4-nitroaniline
-
?
Ala-Pro-Gly + H2O 11% of the activity with Gly-Pro 4-nitroanilide Lactobacillus helveticus Ala-Pro + Gly
-
?
Arg-Pro 4-nitroanilide + H2O 130% of the activity with Gly-Pro 4-nitroanilide Lactobacillus helveticus Arg-Pro + 4-nitroaniline
-
?
Gly-Pro 4-nitroanilide + H2O
-
Lactobacillus helveticus Gly-Pro + 4-nitroaniline
-
?
additional information no activity with Ala-Leu-Ala Lactobacillus helveticus ?
-
?
additional information no activity with Ala-Leu-Ala Lactobacillus helveticus CNRZ 32 ?
-
?

Subunits

Subunits Comment Organism
? x * 72000, native PAGE and SDS-PAGE Lactobacillus helveticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Lactobacillus helveticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 65 10°C: about 55% of activity maximum, 65°C: 34% of activity maximum Lactobacillus helveticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Lactobacillus helveticus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5: about 50% of maximal activity, pH 9: about 60% of maximal activity Lactobacillus helveticus