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Literature summary for 3.4.14.1 extracted from

  • Khaket, T.; Dhanda, S.; Jodha, D.; Singh, J.
    Biochemical studies on dipeptidyl peptidase I (cathepsin C) from germinated Vigna radiata seeds (2016), Process Biochem., 51, 1015-1027 .
No PubMed abstract available
Search for more literature for 3.4.14.1

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 1.5 mM, 81% residual activity Vigna radiata
iodoacetate 1 mM, 59.2% residual activity Vigna radiata
leupeptin 1 mM, 39.1% residual activity Vigna radiata
N-ethylmaleimine 1 mM, 46.4% residual activity Vigna radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 glycine-L-arginine-4-methoxy-beta-naphthylamide pH 7.0, 40°C Vigna radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1 mM, 123% of initial activity Vigna radiata
Fe2+ 0.2 mM, 127% of initial activity Vigna radiata
K+ 0.1 mM, 141% of initial activity Vigna radiata
Mg2+ 0.1 mM, 141% of initial activity Vigna radiata
Mn2+ 0.1 mM, 125% of initial activity Vigna radiata
NH4+ 0.2 mM, 132% of initial activity Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 PAGE Vigna radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from germinated seed Vigna radiata

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Vigna radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
361
-
 pH 7.0, 40°C Vigna radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine-L-alanine-4-methoxy-beta-naphthylamide + H2O 56.7% of the activity with glycine-L-arginine-4-methoxy-beta-naphthylamide Vigna radiata glycine-L-alanine + 4-methoxy-beta-naphthylamine
-
 ?
glycine-L-arginine-4-methoxy-beta-naphthylamide + H2O
-
 Vigna radiata glycine-L-arginine + 4-methoxy-beta-naphthylamine
-
 ?
glycine-L-arginine-beta-naphthylamide + H2O 90.1% of the activity with glycine-L-arginine-4-methoxy-beta-naphthylamide Vigna radiata glycine-L-arginine + beta-naphthylamine
-
 ?
glycine-L-phenylalanine-beta-naphthylamide + H2O 96.5% of the activity with glycine-L-arginine-4-methoxy-beta-naphthylamide Vigna radiata glycine-L-phenylalanine + beta-naphthylamine
-
 ?
L-phenylalanine-L-arginine-4-methoxy-beta-naphthylamide + H2O 87.2% of the activity with glycine-L-arginine-4-methoxy-beta-naphthylamide Vigna radiata L-phenylalanine-L-arginine + 4-methoxy-beta-naphthylamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE, 1 * 38010, MALDI-TOF Vigna radiata

Synonyms

Synonyms Comment Organism
DPP-I
-
 Vigna radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Vigna radiata

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 more than 70% of maximum activity Vigna radiata
8
-
 more than 70% of maximum activity Vigna radiata