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Literature summary for 3.4.13.9 extracted from

  • Suga, K.; Kabashima, T.; Ito, K.; Tsuru, D.; Okamura, H.; Kataoka, J.; Yoshimoto, T.
    Prolidase from Xanthomonas maltophilia: purification and characterization of the enzyme (1995), Biosci. Biotechnol. Biochem., 59, 2087-2090.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
diethyldithiocarbamate activation Stenotrophomonas maltophilia
EDTA activation Stenotrophomonas maltophilia
EGTA activation Stenotrophomonas maltophilia

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Stenotrophomonas maltophilia
2-mercaptoethanol weakly Stenotrophomonas maltophilia
Cu2+
-
Stenotrophomonas maltophilia
Hg2+
-
Stenotrophomonas maltophilia
p-chloromercuribenzoate
-
Stenotrophomonas maltophilia
phenylacetyl-thioproline
-
Stenotrophomonas maltophilia
Z-L-proline
-
Stenotrophomonas maltophilia
Zn2+
-
Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulation Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
2 * 51000, SDS-PAGE Stenotrophomonas maltophilia
100000
-
gel filtration Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Stenotrophomonas maltophilia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.35
-
-
Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
His-Pro + H2O
-
Stenotrophomonas maltophilia His + Pro
-
?
Ile-Pro + H2O
-
Stenotrophomonas maltophilia Ile + Pro
-
?
L-Ala-Pro + H2O
-
Stenotrophomonas maltophilia L-Ala + Pro
-
?
L-Phe-Pro + H2O
-
Stenotrophomonas maltophilia L-Phe + Pro
-
?
L-Val-Pro + H2O
-
Stenotrophomonas maltophilia L-Val + Pro
-
?
Leu-Pro + H2O
-
Stenotrophomonas maltophilia Leu + Pro
-
?
Met-Pro + H2O
-
Stenotrophomonas maltophilia Met + Pro
-
?
Pro-Pro + H2O
-
Stenotrophomonas maltophilia Pro + Pro
-
?
Ser-Pro + H2O
-
Stenotrophomonas maltophilia Ser + Pro
-
?
Thr-Pro + H2O
-
Stenotrophomonas maltophilia Thr + Pro
-
?
Tyr-Pro + H2O
-
Stenotrophomonas maltophilia Tyr + Pro
-
?

Subunits

Subunits Comment Organism
dimer 2 * 51000, SDS-PAGE Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Stenotrophomonas maltophilia

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.5 for 60 min at 37°C Stenotrophomonas maltophilia