Literature summary for 3.4.13.9 extracted from

Morel, F.; Frot-Coutaz, F.; Aubel, D.; Portalier, R.; Atlan, D.
Characterization of a prolidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 with an unusual regulation of biosynthesis (1999), Microbiology, 145, 437-446.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Lactobacillus delbrueckii

Inhibitors

Inhibitors	Comment	Organism	Structure
bestatin	-	Lactobacillus delbrueckii
dithiothreitol	-	Lactobacillus delbrueckii
EDTA	-	Lactobacillus delbrueckii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.2	-	Leu-Pro	-	Lactobacillus delbrueckii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	requirement	Lactobacillus delbrueckii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	2 * 50000, SDS-PAGE	Lactobacillus delbrueckii
68000	70000	gel filtration	Lactobacillus delbrueckii

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus delbrueckii	-	subsp. bulgaricus CNRZ 397	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lactobacillus delbrueckii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
446	-	-	Lactobacillus delbrueckii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Arg-Pro + H2O	-	Lactobacillus delbrueckii	Arg + Pro	-	?
L-Ala-Pro + H2O	-	Lactobacillus delbrueckii	L-Ala + Pro	-	?
L-Phe-Pro + H2O	-	Lactobacillus delbrueckii	L-Phe + Pro	-	?
L-Val-Pro + H2O	-	Lactobacillus delbrueckii	L-Val + Pro	-	?
Leu-Pro + H2O	-	Lactobacillus delbrueckii	Leu + Pro	-	?
additional information	no substrate: Pro-Pro, Gly-Pro	Lactobacillus delbrueckii	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 50000, SDS-PAGE	Lactobacillus delbrueckii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Lactobacillus delbrueckii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Lactobacillus delbrueckii

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Release 2023.1 (January 2023)