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Literature summary for 3.4.13.22 extracted from

  • Breece, R.M.; Costello, A.; Bennett, B.; Sigdel, T.K.; Matthews, M.L.; Tierney, D.L.; Crowder, M.W.
    A five-coordinate metal center in Co(II)-substituted VanX (2005), J. Biol. Chem., 280, 11074-11081.
    View publication on PubMed
Search for more literature for 3.4.13.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 D-Ala-D-Ala maltose-binding protein fusion enzyme, Co2+-form, pH 8.0, 37°C Enterococcus faecium
0.109
-
 D-Ala-D-Ala Zn2+-form, pH 8.0, 37°C Enterococcus faecium
0.14
-
 D-Ala-D-Ala Co2+-form, pH 8.0, 37°C Enterococcus faecium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+ is five-coordinate and has at least one solvent-derived ligand. About 0.7 equivalents per molecule Enterococcus faecium
additional information no other metal ions than Co2+ or Zn2+ found Enterococcus faecium
Zn2+ may substitute for Co2+. About 0.7 equivalents per molecule Enterococcus faecium

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
 recombinant enzyme, Co2+-form
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-D-Ala + H2O
-
 Enterococcus faecium D-Ala + D-Ala
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
94
-
 D-Ala-D-Ala Co2+-form, pH 8.0, 37°C Enterococcus faecium
110
-
 D-Ala-D-Ala maltose-binding protein fusion enzyme, Co2+-form, pH 8.0, 37°C Enterococcus faecium
156
-
 D-Ala-D-Ala Zn2+-form, pH 8.0, 37°C Enterococcus faecium