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Literature summary for 3.4.13.22 extracted from
- Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147 (1995), Biochemistry, 34, 2455-2463.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme may be a target for drug design to reverse clinical vancomycin resistance | Enterococcus faecium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene vanX, overexpression of the soluble enzyme in Escherichia coli | Enterococcus faecium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Ala-D-lactate | very poor hydrolysis, blocking of the enzyme | Enterococcus faecium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
D-Ala-D-Ala | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 1.2 | - |
Co2+ | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 1.7 | - |
D-Ser-D-Ala | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 2.8 | - |
D-Ala-D-Ser | pH 7.0-9.0, 37°C | Enterococcus faecium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates, optimal at 2 mM | Enterococcus faecium | |
| Fe2+ | highly stimulating, optimal at 3 mM | Enterococcus faecium | |
| additional information | effect of diverse metal ions on enzyme activity, overview | Enterococcus faecium | |
| Ni2+ | highly stimulating, optimal at 1 mM | Enterococcus faecium | |
| Zn2+ | activates, optimal at 0.5 mM | Enterococcus faecium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24200 | - |
x * 24200, recombinant enzyme, SDS-PAGE | Enterococcus faecium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala-D-Ala + H2O | Enterococcus faecium | enzyme is essential for vancomycin resistance in Enterococcus faecium | D-Ala + D-Ala | - |
? | |
| additional information | Enterococcus faecium | substrate specificty, no activity with D-Ala-D-lactate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecium | Q06241 | i.e. Streptococcus faecium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble recombinant enzyme from Escherichia coli, to homogeneity | Enterococcus faecium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Enterococcus faecium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala-D-Ala + H2O | - |
Enterococcus faecium | D-Ala + D-Ala | - |
? | |
| D-Ala-D-Ala + H2O | enzyme is essential for vancomycin resistance in Enterococcus faecium | Enterococcus faecium | D-Ala + D-Ala | - |
? | |
| D-Ala-D-Phe + H2O | - |
Enterococcus faecium | D-Ala + D-Phe | - |
? | |
| D-Ala-D-Ser + H2O | - |
Enterococcus faecium | D-Ala + D-Ser | - |
? | |
| D-Ala-Gly + H2O | - |
Enterococcus faecium | D-Ala + Gly | - |
? | |
| D-Ser-D-Ala + H2O | - |
Enterococcus faecium | D-Ser + D-Ala | - |
? | |
| DL-Ala-DL-Asn + H2O | - |
Enterococcus faecium | DL-Ala + DL-Asn | - |
? | |
| DL-Ala-DL-Ser + H2O | - |
Enterococcus faecium | DL-Ala + DL-Ser | - |
? | |
| DL-Ala-DL-Val + H2O | - |
Enterococcus faecium | DL-Ala + DL-Val | - |
? | |
| additional information | substrate specificty, no activity with D-Ala-D-lactate | Enterococcus faecium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24200, recombinant enzyme, SDS-PAGE | Enterococcus faecium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-, D-dipeptidase | - |
Enterococcus faecium |
| D-Ala-D-Ala-dipeptidase | - |
Enterococcus faecium |
| M15.011 | Merops-ID | Enterococcus faecium |
| Vancomycin B-type resistance protein vanX | - |
Enterococcus faecium |
| VanX | - |
Enterococcus faecium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
D-Ser-D-Ala | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 1.8 | - |
D-Ala-D-Ser | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 4.7 | - |
D-Ala-D-Ala | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 30 | - |
Zn2+ | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 69 | - |
Co2+ | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 156 | - |
Fe2+ | pH 7.0-9.0, 37°C | Enterococcus faecium | |
| 788 | - |
Ni2+ | pH 7.0-9.0, 37°C | Enterococcus faecium | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Enterococcus faecium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 242 | - |
D-Ala-D-lactate | pH 7.0-9.0, 37°C | Enterococcus faecium | |
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