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Literature summary for 3.4.13.21 extracted from

  • Kira, I.; Asano, Y.; Yokozeki, K.
    Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from l-isoasparagine and L-phenylalanine methyl ester (2009), J. Biosci. Bioeng., 108, 190-193.
    View publication on PubMed
Search for more literature for 3.4.13.21

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Cedecea lapagei
-
-
-
Cedecea lapagei TPU 5750
-
-
-
Escherichia coli
-
-
-
Escherichia coli TPU 6303
-
-
-
Hafnia alvei
-
-
-
Hafnia alvei TPU 6440
-
-
-
Klebsiella aerogenes
-
-
-
Klebsiella aerogenes TPU 6151
-
-
-
Morganella morganii
-
-
-
Morganella morganii TPU 6501
-
-
-
Rahnella aquatilis
-
-
-
Rahnella aquatilis TPU 5901
-
-
-
Raoultella planticola
-
-
-
Raoultella planticola TPU 6501
-
-
-
Serratia marcescens
-
-
-
Serratia marcescens TPU 7303
-
-
-
Yersinia aldovae
-
-
-
Yersinia aldovae TPU 7650
-
-
-

Purification (Commentary)

Purification (Comment) Organism
yield of 1.2% after 5-20% gradient acrylamide slab gel Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.39
-
 purified enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Escherichia coli N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Cedecea lapagei N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Klebsiella aerogenes N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Hafnia alvei N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Raoultella planticola N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Morganella morganii N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Rahnella aquatilis N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Serratia marcescens N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Yersinia aldovae N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Cedecea lapagei TPU 5750 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Klebsiella aerogenes TPU 6151 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Escherichia coli TPU 6303 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Hafnia alvei TPU 6440 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Morganella morganii TPU 6501 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Raoultella planticola TPU 6501 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Rahnella aquatilis TPU 5901 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Serratia marcescens TPU 7303 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?
L-isoasparagine + L-phenylalanine methyl ester assay at 30°C, pH 10.0 Yersinia aldovae TPU 7650 N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester + ?
-
 ?

Synonyms

Synonyms Comment Organism
dipeptidase E
-
 Escherichia coli
dipeptidase E
-
 Cedecea lapagei
dipeptidase E
-
 Klebsiella aerogenes
dipeptidase E
-
 Hafnia alvei
dipeptidase E
-
 Raoultella planticola
dipeptidase E
-
 Morganella morganii
dipeptidase E
-
 Rahnella aquatilis
dipeptidase E
-
 Serratia marcescens
dipeptidase E
-
 Yersinia aldovae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Cedecea lapagei
30
-
 assay at Escherichia coli
30
-
 assay at Hafnia alvei
30
-
 assay at Klebsiella aerogenes
30
-
 assay at Morganella morganii
30
-
 assay at Rahnella aquatilis
30
-
 assay at Raoultella planticola
30
-
 assay at Serratia marcescens
30
-
 assay at Yersinia aldovae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 assay at Escherichia coli
10
-
 assay at Cedecea lapagei
10
-
 assay at Klebsiella aerogenes
10
-
 assay at Hafnia alvei
10
-
 assay at Raoultella planticola
10
-
 assay at Morganella morganii
10
-
 assay at Rahnella aquatilis
10
-
 assay at Serratia marcescens
10
-
 assay at Yersinia aldovae