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Literature summary for 3.4.13.21 extracted from

  • Conlin, C.A.; Hakensson, K.; Liljas, A.; Miller, C.G.
    Cloning and nucleotide sequence of the cyclic AMP receptor protein-regulated Salmonella typhimurium pepE gene and crystallization of its product, an alpha-aspartyl dipeptidase (1994), J. Bacteriol., 176, 166-172.
    View publication on PubMed
Search for more literature for 3.4.13.21

Cloned(Commentary)

Cloned (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
beta-Alanine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
L-Asp
-
 Salmonella enterica subsp. enterica serovar Typhimurium
additional information not inhibited by EDTA, dipicolinic acid, DL-2-amino-2-methylpropanoate Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3
-
 L-Asp-4-nitroanilide
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not contain zinc or any other metals Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24770
-
 calculation from sequence of cDNA Salmonella enterica subsp. enterica serovar Typhimurium
27000
-
 SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
 GenBank accession number U01246
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp-4-nitroanilide + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-Asp + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39
-
 L-Asp-4-nitroanilide
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
 Salmonella enterica subsp. enterica serovar Typhimurium