Literature summary for 3.4.13.20 extracted from

Peters, V.; Schmitt, C.P.; Weigand, T.; Klingbeil, K.; Thiel, C.; van den Berg, A.; Calabrese, V.; Nawroth, P.; Fleming, T.; Forsberg, E.; Wagner, A.H.; Hecker, M.; Vistoli, G.
Allosteric inhibition of carnosinase (CN1) by inducing a conformational shift (2017), J. Enzyme Inhib. Med. Chem., 32, 1102-1110 .

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Protein Variants

Protein Variants	Comment	Organism
C102S	efficiency for carnosine degradation is not influenced by the addition of cysteine	Mus musculus
C229S	addition of cysteine to C229S significantly reduces CN1 catalytic efficiency	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
cysteine	lowers dose-dependently recombinant CN1 efficiency	Mus musculus
N-acetylcysteine	lowers dose-dependently recombinant CN1 efficiency	Mus musculus
reduced glutathione	lowers dose-dependently recombinant CN1 efficiency	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.7	-	carnosine	pH 7.0, temperature not specified in the publication, presence of 1 mM glycine	Mus musculus
0.8	-	carnosine	pH 7.0, temperature not specified in the publication	Mus musculus
0.8	-	carnosine	pH 7.0, temperature not specified in the publication, presence of 1 mM L-glutamate	Mus musculus
0.9	-	carnosine	pH 7.0, temperature not specified in the publication, presence of 1 mM glutathione	Mus musculus
0.9	-	carnosine	pH 7.0, temperature not specified in the publication, presence of 1 mM reduced glutathione	Mus musculus
1	-	carnosine	pH 7.0, temperature not specified in the publication, presence of 1 mM cysteine	Mus musculus
1.1	-	carnosine	pH 7.0, temperature not specified in the publication, presence of 1 mM N-acetylcysteine	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q8BUG2	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carnosine + H2O	-	Mus musculus	beta-Ala + His	-	?

Synonyms

Synonyms	Comment	Organism
CN1	-	Mus musculus
CNDP1	-	Mus musculus

General Information

General Information	Comment	Organism
physiological function	reduced glutathione, N-acetylcysteine and cysteine lower dose-dependently recombinant CN1 efficiency and normalize increased CN1 activity in renal tissue samples of diabetic mice. Inhibition is allosteric. Only cysteine-102 is influenced by cysteinylation	Mus musculus

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Release 2023.1 (January 2023)