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Literature summary for 3.4.13.20 extracted from

  • Komeda, H.; Asano, Y.
    A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase specific for beta-alanyl dipeptides (2005), FEBS J., 272, 3075-3084.
    View publication on PubMed
Search for more literature for 3.4.13.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 30°C, 10 min, 1 mM, 95% loss of activity Pseudomonas sp.
CdCl2 30°C, 10 min, 1 mM, 90% loss of activity Pseudomonas sp.
dithiothreitol 30°C, 10 min, 1 mM, 63% loss of activity Pseudomonas sp.
HgCl2 30°C, 10 min, 1 mM, 99% loss of activity Pseudomonas sp.
additional information no inhibition: o-phenanthroline, 8-hydroxyquinoline, ethylenediaminetetraacetic acid, 2,2'-dipyridyl, hydroxylamine, phenylhydrazine, hydrazine, D,L-penicillamine, D-cycloserine, phenylmethanesulfonyl fluoride, leupeptine, pepstatin, LiCl, H2BO3, NaCl, MgSO4, MgCl2, AlCl3, KCl, CaCl2, CrCl3, MnSO4, MnCl2, FeSO4, FeCl3, CoCl2, NiCl2, CuSO4, CuCl2, RbCl, Na2MoO4 (NH4)6Mo7O24, SnCl2, CsCl, BaCl2 and PbCl2 Pseudomonas sp.
N-ethylmaleimide 30°C, 10 min, 1 mM, 80% loss of activity Pseudomonas sp.
p-chloromercuribenzoate 30°C, 10 min, 1 mM, 95% loss of activity Pseudomonas sp.
ZnCl2 30°C, 10 min, 1 mM, 98% loss of activity Pseudomonas sp.
ZnSO4 30°C, 10 min, 1 mM, 98% loss of activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 alpha4beta4, 2 * 13000 + 2 * 27000, SDS-PAGE Pseudomonas sp.
27000
-
 alpha4beta4, 2 * 13000 + 2 * 27000, SDS-PAGE Pseudomonas sp.
150000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q4R9M3
-
-
Pseudomonas sp. MCI3434 Q4R9M3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the mature enzyme with two polypeptide chains (alpha and beta) is formed by the cleavage of Gly238-Ser239 peptide bond of the 366-residue precursor Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme is purified from the Escherichia coli JM109 harboring p2DAPEX with a recovery of 10.3% Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-Ala-beta-Ala + H2O 48% of the activity compared to beta-Ala-L-Ala Pseudomonas sp. beta-Ala + beta-Ala
-
 ?
beta-Ala-beta-Ala + H2O 48% of the activity compared to beta-Ala-L-Ala Pseudomonas sp. MCI3434 beta-Ala + beta-Ala
-
 ?
beta-Ala-Gly + H2O 76% of the activity compared to beta-Ala-L-Ala Pseudomonas sp. beta-Ala + Gly
-
 ?
beta-Ala-L-Ala + H2O preferred substrate Pseudomonas sp. beta-Ala + L-Ala
-
 ?
beta-Ala-L-His + H2O i.e. carnosine, 57% of the activity compared to beta-Ala-L-Ala Pseudomonas sp. beta-Ala + L-His
-
 ?
beta-Ala-L-His + H2O i.e. carnosine, 57% of the activity compared to beta-Ala-L-Ala Pseudomonas sp. MCI3434 beta-Ala + L-His
-
 ?
beta-Ala-L-Leu + H2O 49% of the activity compared to beta-Ala-L-Ala Pseudomonas sp. beta-Ala + L-Leu
-
 ?
beta-Ala-NH2 + H2O 58% of the activity compared to beta-Ala-L-Ala Pseudomonas sp. beta-Ala + NH3
-
 ?
D-Ala-4-nitroanilide + H2O 16% of the activity compared to beta-Ala-L-Ala. D-Ala-4-nitroanilide is hydrolyzed with 5.8 times high efficiency than L-Ala-4-nitroanilide Pseudomonas sp. D-Ala + 4-nitroaniline
-
 ?
D-Ala-4-nitroanilide + H2O 16% of the activity compared to beta-Ala-L-Ala. D-Ala-4-nitroanilide is hydrolyzed with 5.8 times high efficiency than L-Ala-4-nitroanilide Pseudomonas sp. MCI3434 D-Ala + 4-nitroaniline
-
 ?
D-Ala-NH2 + H2O 0.6% of the activity compared to beta-Ala-L-Ala Pseudomonas sp. D-Ala + NH3
-
 ?
L-Ala-4-nitroanilide + H2O 3% of the activity compared to beta-Ala-L-Ala. D-Ala-4-nitroanilide is hydrolyzed with 5.8 times high efficiency than L-Ala-4-nitroanilide Pseudomonas sp. L-Ala + 4-nitroaniline
-
 ?
L-Ala-4-nitroanilide + H2O 3% of the activity compared to beta-Ala-L-Ala. D-Ala-4-nitroanilide is hydrolyzed with 5.8 times high efficiency than L-Ala-4-nitroanilide Pseudomonas sp. MCI3434 L-Ala + 4-nitroaniline
-
 ?
additional information no activity on the peptides containing proteinogenic amino acids or their D-counterparts for N-terminal residues. gamma-Aminobutyryl-L-His (L-homocarnosine) is not hydrolyzed Pseudomonas sp. ?
-
 ?
additional information no activity on the peptides containing proteinogenic amino acids or their D-counterparts for N-terminal residues. gamma-Aminobutyryl-L-His (L-homocarnosine) is not hydrolyzed Pseudomonas sp. MCI3434 ?
-
 ?

Subunits

Subunits Comment Organism
octamer alpha4beta4, 2 * 13000 + 2 * 27000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
DmpA
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas sp.
60
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, stable below Pseudomonas sp.
50
-
 10 min, 13% loss of activity Pseudomonas sp.
55
-
 10 min, 51% loss of activity Pseudomonas sp.
60
-
 10 min, complete inactivation Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas sp.
9 10
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 30°C, 10 min, stable Pseudomonas sp.