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Literature summary for 3.4.13.20 extracted from

  • Inaba, C.; Higuchi, S.; Morisaka, H.; Kuroda, K.; Ueda, M.
    Synthesis of functional dipeptide carnosine from nonprotected amino acids using carnosinase-displaying yeast cells (2010), Appl. Microbiol. Biotechnol., 86, 1895-1902.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
human CN1 gene is expressed in Saccharomyces cerevisiae on the yeast cell surface with alpha-agglutinin as the anchor protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information using human CN1 gene a whole cell biocatalyst displaying CN1 on the yeast cell surface with alpha-agglutinin as the anchor protein is constructed. CN1-displaying yeast cells show hydrolytic activity for carnosine in hydrophobic organic solvents. CN1-displaying yeast cells can synthesize carnosine from nonprotected amino acids as substrates in hydrophobic organic solvents and in a hydrophobic ionic liquid Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96KN2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
serum cDNA generated from serum Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carnosine + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
CN1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens