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Literature summary for 3.4.13.20 extracted from

  • Jackson, M.C.; Kucera, C.M.; Lenney, J.F.
    Purification and properties of human serum carnosinase (1991), Clin. Chim. Acta, 196, 193-206.
    View publication on PubMed
Search for more literature for 3.4.13.20

General Stability

General Stability Organism
inactivation during DEAE-cellulose chromatography, slow reactivation when concentrated and stored at 4°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 2 * 75000, subunits are connected by one or more disulfide bonds, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
anserine + H2O Homo sapiens splitting in the blood stream beta-Ala + Ntau-methyl-L-histidine
-
 ?
carnosine + H2O Homo sapiens splitting in the blood stream beta-Ala + His
-
 ?
homocarnosine + H2O Homo sapiens splitting of homocarnosine in the brain ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood serum not in serum of other mammals Homo sapiens
-
brain
-
 Homo sapiens
-
additional information the enzyme activity in tissues is roughly proportional to blood content for all the tissues except brain, indicating that serum carnosinase is present in the trapped blood of these tissues Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala + H2O 11% of the activity with carnosine Homo sapiens Ala + Ala
-
 ?
Ala-His + H2O 38% of the activity with carnosine Homo sapiens Ala + His
-
 ?
Ala-Leu + H2O 13% of the activity with carnosine Homo sapiens Ala + Leu
-
 ?
Ala-Tyr + H2O 8% of the activity with carnosine Homo sapiens Ala + Tyr
-
 ?
anserine + H2O 88% of the activity with carnosine Homo sapiens beta-Ala + Ntau-methyl-L-histidine
-
 ?
anserine + H2O splitting in the blood stream Homo sapiens beta-Ala + Ntau-methyl-L-histidine
-
 ?
beta-Ala-Ala + H2O 10% of the activity with carnosine Homo sapiens beta-Ala + Ala
-
 ?
beta-Ala-Phe + H2O 18% of the activity with carnosine Homo sapiens beta-Ala + Phe
-
 ?
carnosine + H2O beta-Ala-His Homo sapiens beta-Ala + His
-
 ?
carnosine + H2O splitting in the blood stream Homo sapiens beta-Ala + His
-
 ?
Gly-Gly + H2O 7% of the activity with carnosine Homo sapiens Gly + Gly
-
 ?
Gly-His + H2O 37% of the activity with carnosine Homo sapiens Gly + His
-
 ?
Gly-His-Gly + H2O 13% of the activity with carnosine Homo sapiens ?
-
 ?
Gly-His-Lys + H2O 7% of the activity with carnosine Homo sapiens ?
-
 ?
Gly-Leu + H2O 21% of the activity with carnosine Homo sapiens Gly + Leu
-
 ?
homocarnosine + H2O 11% of the activity with carnosine Homo sapiens ?
-
 ?
homocarnosine + H2O splitting of homocarnosine in the brain Homo sapiens ?
-
 ?
Leu-Leu + H2O 8% of the activity with carnosine Homo sapiens Leu + Leu
-
 ?
additional information dipeptide with histidine in the C-terminal position is preferred Homo sapiens ?
-
 ?
Phe-Ala + H2O 8% of the activity with carnosine Homo sapiens Phe + Ala
-
 ?
Ser-His + H2O 8% of the activity with carnosine Homo sapiens Ser + His
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 75000, subunits are connected by one or more disulfide bonds, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4
-
-
 Homo sapiens