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Literature summary for 3.4.13.20 extracted from

  • Lenney, J.F.; George, R.P.; Weiss, A.M.; Kucera, C.M.; Chan, P.W.H.; Rinzler, G.S.
    Human serum carnosinase: characterization, distinction from cellular carnosinase, and activation by cadmium (1982), Clin. Chim. Acta, 123, 221-231.
    View publication on PubMed
Search for more literature for 3.4.13.20

Activating Compound

Activating Compound Comment Organism Structure
citrate activates Homo sapiens
phosphate activates Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by p-hydroxymercuribenzoate, CH2ClCOONa, phenylmethylsulfonyl fluoride Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates, twice as effective as Mn2+, maximum activity at 0.8-0.9 mM Homo sapiens
Mn2+ activates, less effective than Cd2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 little or no activity in children below 10 months in age thereafter, the average concentration increases gradually to reach the adult range at the age 13-15
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood serum concentration varies greatly between individuals Homo sapiens
-

Storage Stability

Storage Stability Organism
-80°C, stable for several weeks in serum Homo sapiens
4°C, stable for a day or two in serum Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carnosine + H2O beta-Ala-His Homo sapiens beta-Ala + His
-
 ?
homocarnosine + H2O
-
 Homo sapiens ?
-
 ?
additional information no hydrolysis of L-Ala-His, Gly-His or N-acetylhistidine Homo sapiens ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, pH 7.8, stable, thermostability improved by the presence of 0.25 mM MnCl2, stability is very poor in the presence of 0.25 mM cadmium or calcium Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
-
 Homo sapiens
8 8.5
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8.2 maximal stability at 50°C Homo sapiens