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Literature summary for 3.4.13.19 extracted from
- Glutathione-degrading enzymes of microvillus membranes (1982), J. Biol. Chem., 257, 6322-6327.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | ZnSO4 restores activity | Rattus norvegicus |  |
| DTT | - |
Rattus norvegicus | |
| thiol compounds | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
L-cystinyl-bis-Gly | - |
Rattus norvegicus | |
| 0.8 | - |
L-Ala-Gly | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microvillus | - |
Rattus norvegicus | 5902 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | enzyme contains about 2 Zn per subunit | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, SDS-PAGE | Rattus norvegicus |
| 105000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala + H2O | - |
Rattus norvegicus | Ala + Ala | - |
? | |
| Ala-Met + H2O | - |
Rattus norvegicus | Ala + Met | - |
? | |
| Gly-Ala + H2O | L-Ala and D-Ala | Rattus norvegicus | Gly + Ala | - |
? | |
| Gly-Gly + H2O | - |
Rattus norvegicus | Gly + Gly | - |
? | |
| L-Ala-Gly + H2O | - |
Rattus norvegicus | L-Ala + Gly | - |
? | |
| L-cystinyl-bis-Gly + H2O | - |
Rattus norvegicus | L-Cys + Gly-Gly | - |
? | |
| L-cystinyl-bis-glycine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Lys-Gly + H2O | - |
Rattus norvegicus | Lys + Gly | - |
? | |
| Met-Met + H2O | - |
Rattus norvegicus | Met + Met | - |
? | |
| additional information | no hydrolysis of L-Asp-Gly | Rattus norvegicus | ? | - |
? | |
| additional information | no hydrolysis of proline-containing peptides | Rattus norvegicus | ? | - |
? | |
| additional information | no hydrolysis of glutathione | Rattus norvegicus | ? | - |
? | |
| additional information | no hydrolysis of L-Val-Gly | Rattus norvegicus | ? | - |
? | |
| additional information | no hydrolysis of D-Ala-Gly | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE | Rattus norvegicus |
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Release 2023.1 (January 2023)
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