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Literature summary for 3.4.13.18 extracted from

  • Chen, Y.S.; Chien, C.H.; Goparaju, C.M.; Hsu, J.T.; Liang, P.H.; Chen, X.
    Purification and characterization of human prolyl dipeptidase DPP8 in Sf9 insect cells (2004), Protein Expr. Purif., 35, 142-146.
    View publication on PubMed
Search for more literature for 3.4.13.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Homo sapiens
overexpression in baculovirus infected Sf9 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 Ala-Pro-p-nitroanilide
-
 Homo sapiens
0.31
-
 Arg-Pro-p-nitroanilide
-
 Homo sapiens
0.48
-
 Gly-Pro-p-nitroanilide
-
 Homo sapiens
1.42
-
 Ala-Ala-p-nitroanilide
-
 Homo sapiens
2.05
-
 Asp-Pro-p-nitroanilide
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 8
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-p-nitroanilide + H2O
-
 Homo sapiens Ala-Ala + p-nitroaniline
-
 ?
Ala-Pro-p-nitroanilide + H2O
-
 Homo sapiens Ala-Pro + p-nitroaniline
-
 ?
Arg-Pro-p-nitroanilide + H2O
-
 Homo sapiens Arg-Pro + p-nitroaniline
-
 ?
Asp-Pro-p-nitroanilide + H2O
-
 Homo sapiens Asp-Pro + p-nitroaniline
-
 ?
Gly-Pro-p-nitroanilide + H2O
-
 Homo sapiens Gly-Pro + p-nitroaniline
-
 ?
additional information the enzyme catalyzes cleavage at the carboxyl side of the proline residue at the penultimate position Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
DPP8
-
 Homo sapiens
prolyl dipeptidase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 Ala-Ala-p-nitroanilide
-
 Homo sapiens
1.6
-
 Asp-Pro-p-nitroanilide
-
 Homo sapiens
12.8
-
 Arg-Pro-p-nitroanilide
-
 Homo sapiens
40
-
 Ala-Pro-p-nitroanilide
-
 Homo sapiens
62
-
 Gly-Pro-p-nitroanilide
-
 Homo sapiens