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Literature summary for 3.4.13.18 extracted from
- A dipeptidase from Ehrlich Lettre mouse ascites tumor cells (1976), Methods Enzymol., 45, 386-393.
General Stability
| General Stability | Organism |
|---|---|
| addition of 2 mg/ml bovine serum albumin stabilizes the enzyme for assay for 5 min at 40°C | Mus musculus |
| sucrose is necessary for stability | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Mus musculus |  |
| 2-mercaptoethanol | - |
Mus musculus | |
| D-Forms of dipeptides | - |
Mus musculus | |
| DTT | - |
Mus musculus | |
| EDTA | - |
Mus musculus | |
| L-alanine | poor competitive inhibitor of Ala-Gly hydrolysis | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Mus musculus | |
| Mn2+ | activates | Mus musculus | |
| Zn2+ | possibly contains 1 mol of Zn2+ per mol of enzyme | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000, SDS-PAGE | Mus musculus |
| 85000 | - |
gel filtration | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ascites tumor cell | Ehrlich Lettre | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, 0.02 M potassium phosphate, pH 8.2, 0.25 M sucrose, stable | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala-Gly + H2O | - |
Mus musculus | L-Ala + Gly | - |
? | |
| additional information | broad specificity | Mus musculus | ? | - |
? | |
| additional information | specificity overview | Mus musculus | ? | - |
? | |
| additional information | not: Lys-Gly | Mus musculus | ? | - |
? | |
| additional information | not: Gly-Asp | Mus musculus | ? | - |
? | |
| additional information | not: Asp-Gly | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, SDS-PAGE | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Mus musculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | stable | Mus musculus |
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Release 2023.1 (January 2023)
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