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Literature summary for 3.4.13.12 extracted from

  • Woessner, J.F.
    Met-X dipeptidase (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 1, 1018-1020.
No PubMed abstract available
Search for more literature for 3.4.13.12

General Stability

General Stability Organism
unstable in absence of metal ions and reducing agents. Mn2+ is the best cation for maintaining stability Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid
-
 Escherichia coli
PCMB inhibition is reversed by 2-mercaptoethanol Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ is the only divalent cation to restore activity following EDTA treatment Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, SDS-PAGE Escherichia coli
94000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Ala + H2O cleaved most rapidly Escherichia coli Met + Ala
-
 ?
Met-Glu + H2O cleaved at about 10% of the maximal rate Escherichia coli Met + Glu
-
 ?
Met-Gly + H2O cleaved at about 60% of the maximal rate Escherichia coli Met + Gly
-
 ?
Met-Ile + H2O cleaved at about 10% of the maximal rate Escherichia coli Met + Ile
-
 ?
Met-Leu + H2O cleaved at about 60% of the maximal rate Escherichia coli Met + Leu
-
 ?
Met-Ser + H2O cleaved most rapidly Escherichia coli Met + Ser
-
 ?
Met-Thr + H2O cleaved at about 60% of the maximal rate Escherichia coli Met + Thr
-
 ?
Met-Val + H2O cleaved at about 10% of the maximal rate Escherichia coli Met + Val
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 9
-
 Escherichia coli