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Literature summary for 3.4.13.12 extracted from
- Partial purification and characterization of two peptidases from Neurospora crassa (1974), Biochim. Biophys. Acta, 370, 530-540.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Neurospora crassa |  |
| p-chloromercuribenzoate | 100% inhibition at 1 mM | Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | 0.31 | Met-Ala | - |
Neurospora crassa | |
| 0.42 | 1.02 | Met-Ile | - |
Neurospora crassa | |
| 0.57 | 0.6 | Met-Ser | - |
Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 52% of activity with manganese | Neurospora crassa | |
| Fe2+ | 21% of activity with manganese | Neurospora crassa | |
| Mn2+ | - |
Neurospora crassa | |
| Zn2+ | 20% of activity with manganese | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | 114000 | gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Met-amino acid + H2O | Neurospora crassa | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
- |
Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala + H2O | - |
Neurospora crassa | Ala + Ala | - |
ir | |
| Ala-Leu + H2O | - |
Neurospora crassa | Ala + Leu | - |
ir | |
| Ala-Met + H2O | - |
Neurospora crassa | Ala + Met | - |
ir | |
| ethionyl-Ala + H2O | - |
Neurospora crassa | ethionine + Ala | - |
ir | |
| Leu-Ala + H2O | - |
Neurospora crassa | Leu + Ala | - |
ir | |
| Met-Ala + H2O | best substrate | Neurospora crassa | Met + Ala | - |
ir | |
| Met-amino acid + H2O | - |
Neurospora crassa | ? | - |
? | |
| Met-Ile + H2O | - |
Neurospora crassa | Met + Ile | - |
ir | |
| Met-Leu + H2O | - |
Neurospora crassa | Met + Leu | - |
ir | |
| Met-Met + H2O | - |
Neurospora crassa | Met + Met | - |
ir | |
| Met-Ser + H2O | - |
Neurospora crassa | Met + Ser | - |
ir | |
| Met-Thr + H2O | - |
Neurospora crassa | Met + Thr | - |
ir | |
| norleucyl-Ala + H2O | - |
Neurospora crassa | norleucine + Ala | - |
ir | |
| Ser-Ala + H2O | - |
Neurospora crassa | Ser + Ala | - |
ir | |
| Thr-Ala + H2O | - |
Neurospora crassa | Thr + Ala | - |
ir | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
substrate Met-Ala | Neurospora crassa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | substrate Met-Ala | Neurospora crassa |
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Release 2023.1 (January 2023)
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