Literature summary for 3.4.11.B9 extracted from

Dura, M.A.; Receveur-Brechot, V.; Andrieu, J.P.; Ebel, C.; Schoehn, G.; Roussel, A.; Franzetti, B.
Characterization of a TET-like aminopeptidase complex from the hyperthermophilic archaeon Pyrococcus horikoshii (2005), Biochemistry, 44, 3477-3486 .

Search for more literature for 3.4.11.B9

Activating Compound

Activating Compound	Comment	Organism	Structure
Co2+	activates	Pyrococcus horikoshii

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrococcus horikoshii

Inhibitors

Inhibitors	Comment	Organism
bestatin	-	Pyrococcus horikoshii
EDTA	-	Pyrococcus horikoshii
HEPES	-	Pyrococcus horikoshii
additional information	the enzyme is insensitive to the papain and trypsin inhibitor antipain, to the chymotrypsin inhibitor chymostatin, to the cysteine protease inhibitor E-64, to the serine and cysteine protease inhibitor leupeptin, to the aspartate protease inhibitor pepstatin, and to the serine protease inhibitors Pefabloc SC and aprotinin. Puromycin, an inhibitor of some exopeptidases, has no effect on the activity of PhTET2	Pyrococcus horikoshii
phosphoramidon	weak inhibition	Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
475000	-	tetrahedral dodecameric complex, sedimentation velocity experiments	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O59196	-	-
Pyrococcus horikoshii OT-3	O59196	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
Ala-Ala 4-nitroanilide + H2O	2.9% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Ala-Ala + 4-nitroaniline	-	?
Ala-Ala 4-nitroanilide + H2O	2.9% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii OT-3	Ala-Ala + 4-nitroaniline	-	?
Ala-Ala-Ala 4-nitroanilide + H2O	1.3% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Ala-Ala-Ala + 4-nitroaniline	-	?
Ala-Ala-Phe 4-nitroanilide + H2O	1.2% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Ala-Ala-Phe + 4-nitroaniline	-	?
Asn-7-amido-4-methylcoumarin + H2O	0.3% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Asn + 7-amino-4-methylcoumarin	-	?
Gln-7-amido-4-methylcoumarin + H2O	2.8% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Gln + 7-amino-4-methylcoumarin	-	?
Gln-7-amido-4-methylcoumarin + H2O	2.8% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii OT-3	Gln + 7-amino-4-methylcoumarin	-	?
glycyl 4-nitroanilide + H2O	0.3% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	glycine + 4-nitroaniline	-	?
L-alanyl 4-nitroanilide + H2O	14.3% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	L-alanine + 4-nitroaniline	-	?
L-arginyl 4-nitroanilide + H2O	0.1% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	L-arginine + 4-nitroaniline	-	?
L-isoleucyl 4-nitroanilide + H2O	10.8% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	L-isoleucine + 4-nitroaniline	-	?
L-leucyl 4-nitroanilide + H2O	-	Pyrococcus horikoshii	L-leucine + 4-nitroaniline	-	?
L-lysyl 4-nitroanilide + H2O	0.2% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	L-lysine + 4-nitroaniline	-	?
L-methionyl 4-nitroanilide + H2O	26.3% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	L-methionine + 4-nitroaniline	-	?
L-phenylalanyl 4-nitroanilide + H2O	0.8% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	L-phenylalanine + 4-nitroaniline	-	?
L-prolyl 4-nitroanilide + H2O	0.9% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	L-proline + 4-nitroaniline	-	?
additional information	when assayed with short chromogenic peptides, PhTET2 is active as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is found to be nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity	Pyrococcus horikoshii	?	-	?
additional information	when assayed with short chromogenic peptides, PhTET2 is active as an aminopeptidase, with a clear preference for leucine as the N-terminal amino acid. The enzyme can cleave moderately long polypeptide substrates of various compositions in a fairly unspecific manner. The hydrolytic mechanism is found to be nonprocessive. The enzyme has neither carboxypeptidase nor endoproteolytic activities, and it is devoid of N-terminal deblocking activity	Pyrococcus horikoshii OT-3	?	-	?
Orn-7-amido-4-methylcoumarin + H2O	0.1% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Orn + 7-amino-4-methylcoumarin	-	?
Ser-7-amido-4-methylcoumarin + H2O	12.8% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Ser + 7-amino-4-methylcoumarin	-	?
Ser-7-amido-4-methylcoumarin + H2O	12.8% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii OT-3	Ser + 7-amino-4-methylcoumarin	-	?
Thr-7-amido-4-methylcoumarin + H2O	6.1% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Thr + 7-amino-4-methylcoumarin	-	?
Tyr-7-amido-4-methylcoumarin + H2O	0.9% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Tyr + 7-amino-4-methylcoumarin	-	?
Tyr-7-amido-4-methylcoumarin + H2O	0.9% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii OT-3	Tyr + 7-amino-4-methylcoumarin	-	?
Val-7-amido-4-methylcoumarin + H2O	2.1% of the activity with Leu-4-nitroanilide	Pyrococcus horikoshii	Val + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
dodecamer	12 * 39014, calculated from sequence	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
PH1527	locus name	Pyrococcus horikoshii
PhTET2	-	Pyrococcus horikoshii
tetrahedral aminopeptidase 2	-	Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
100	-	-	Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
70	-	at temperatures lower than 70 °C, less than 10% of the maximum activity was detected	Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	half-life: 10.03 h	Pyrococcus horikoshii
90	-	half-life: 3.00 h	Pyrococcus horikoshii
95	-	half-life: 59.4 min	Pyrococcus horikoshii
100	-	half-life: 24.8 min	Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	substrate: Leu-4-nitroanilide, PIPES buffer	Pyrococcus horikoshii

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	substrate: Leu-4-nitroanilide, PIPES buffer, strong activity was still detectable at pH 6 and 9	Pyrococcus horikoshii