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Literature summary for 3.4.11.9 extracted from

  • Arima, J.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
    Streptomyces aminopeptidase P: biochemical characterization and insight into the roles of its N-terminal domain (2008), Protein Eng. Des. Sel., 21, 45-53.
    View publication on PubMed
Search for more literature for 3.4.11.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces lividans
expression in Escherichia coli Streptomyces murinus

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric enzymes th-sl cAPP, in which half of the N-terminal domain is derived from Streptomyces costaricanus and the rest is Streptomyces lividans isoform II and sl-th cAPP, in which half of the N-terminal domain is Streptomyces lividans isoform II and the rest is Streptomyces costaricanus. Both chimera are activated by Zn2+. Th-sl cAPP and sl-th cAPP, respectively, form a tetramer and a dimer. The pH dependence and pKa values of th-sl cAPP were almost identical to those of Streptomyces costaricanus. In contrast, sl-th cAPP showed curves and pKa values that resembled those of Streptomyces lividans isoform II Streptomyces lividans
additional information construction of chimeric enzymes th-sl cAPP, in which half of the N-terminal domain is derived from Streptomyces costaricanus and the rest is Streptomyces lividans isoform II and sl-th cAPP, in which half of the N-terminal domain is Streptomyces lividans isoform II and the rest is Streptomyces costaricanus. Both chimera are activated by Zn2+. Th-sl cAPP and sl–th cAPP, respectively, form a tetramer and a dimer. The pH dependence and pKa values of th-sl cAPP are almost identical to those of Streptomyces costaricanus. In contrast, sl-th cAPP shows curves and pKa values that resemble those of Streptomyces lividans isoform II Streptomyces murinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
 L-Ala-L-Pro-4-nitroanilide chimera th-sl cAPP, pH 7.0, 30°C Streptomyces lividans
0.51
-
 L-Ala-L-Pro-4-nitroanilide chimera th-sl cAPP, pH 7.0, 30°C Streptomyces murinus
1.4
-
 L-Ala-L-Pro-4-nitroanilide recombinant enzyme, pH 7.4, 30°C Streptomyces lividans
1.5
-
 L-Ala-L-Pro-4-nitroanilide native enzyme, pH 7.4, 30°C Streptomyces murinus
1.7
-
 L-Ala-L-Pro-4-nitroanilide recombinant enzyme, pH 7.4, 30°C Streptomyces murinus
16.9
-
 L-Ala-L-Pro-4-nitroanilide chimera sl-th cAPP, pH 8.2, 30°C Streptomyces lividans
16.9
-
 L-Ala-L-Pro-4-nitroanilide chimera sl-th cAPP, pH 8.2, 30°C Streptomyces murinus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ activation Streptomyces murinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 4 * 55000, SDS-PAGE Streptomyces murinus
220000
-
 gel filtration Streptomyces murinus

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
 isoform APP II
-
Streptomyces murinus A9E3K0
-
-
Streptomyces murinus TH-4 A9E3K0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-L-Pro-4-nitroanilide + H2O best substrate Streptomyces lividans L-Ala + L-Pro-4-nitroanilide
-
 ?
L-Ala-L-Pro-4-nitroanilide + H2O best substrate Streptomyces murinus L-Ala + L-Pro-4-nitroanilide
-
 ?
L-Ala-L-Pro-4-nitroanilide + H2O best substrate Streptomyces murinus TH-4 L-Ala + L-Pro-4-nitroanilide
-
 ?
L-Met-L-Pro + H2O
-
 Streptomyces murinus L-Met + L-Pro
-
 ?
L-Met-L-Pro + H2O
-
 Streptomyces murinus TH-4 L-Met + L-Pro
-
 ?
L-Met-L-Pro-Gly + H2O
-
 Streptomyces murinus L-Met + L-Pro-Gly
-
 ?
L-Met-L-Pro-Gly + H2O
-
 Streptomyces murinus TH-4 L-Met + L-Pro-Gly
-
 ?
L-Phe-L-Pro-Gly + H2O
-
 Streptomyces murinus L-Phe + L-Pro-Gly
-
 ?
L-Phe-L-Pro-Gly + H2O
-
 Streptomyces murinus TH-4 L-Phe + L-Pro-Gly
-
 ?
L-Phe-L-Pro-L-Ala + H2O
-
 Streptomyces murinus L-Phe + L-Pro-L-Ala
-
 ?
L-Phe-L-Pro-L-Ala + H2O
-
 Streptomyces murinus TH-4 L-Phe + L-Pro-L-Ala
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Streptomyces murinus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 40
-
 Streptomyces murinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
32
-
 stable up to Streptomyces murinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
 L-Ala-L-Pro-4-nitroanilide chimera th-sl cAPP, pH 7.0, 30°C Streptomyces lividans
1.5
-
 L-Ala-L-Pro-4-nitroanilide chimera th-sl cAPP, pH 7.0, 30°C Streptomyces murinus
6
-
 L-Ala-L-Pro-4-nitroanilide chimera sl-th cAPP, pH 8.2, 30°C Streptomyces lividans
6
-
 L-Ala-L-Pro-4-nitroanilide chimera sl-th cAPP, pH 8.2, 30°C Streptomyces murinus
45.7
-
 L-Ala-L-Pro-4-nitroanilide recombinant enzyme, pH 7.4, 30°C Streptomyces lividans
398
-
 L-Ala-L-Pro-4-nitroanilide recombinant enzyme, pH 7.4, 30°C Streptomyces murinus
487
-
 L-Ala-L-Pro-4-nitroanilide native enzyme, pH 7.4, 30°C Streptomyces murinus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
 Streptomyces murinus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
 Streptomyces murinus