General Stability | Organism |
---|---|
polyethylene glycol 20000 improves stability in the pH-range 4.5-7.0 | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Rattus norvegicus | |
2-mercaptoethanol | - |
Rattus norvegicus | |
2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | - |
Rattus norvegicus | |
4-chloromercuriphenyl sulfonic acid | - |
Rattus norvegicus | |
apstatin | - |
Rattus norvegicus | |
captopril | - |
Rattus norvegicus | |
Co2+ | 1.5 mM CoCl2, complete inhibition | Rattus norvegicus | |
dithiothreitol | - |
Rattus norvegicus | |
EDTA | - |
Rattus norvegicus | |
enalaprilat | inhibition only in presence of Mn2+ | Rattus norvegicus | |
N-[1-(R,S)-carboxy-(2-phenylethyl)]-thiopropanoic acid | - |
Rattus norvegicus | |
NaCl | 2 M, complete inhibition | Rattus norvegicus | |
ramiprilat | inhibition only in presence of Mn2+ | Rattus norvegicus | |
Zn2+ | 10 mM, complete inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
bradykinin | - |
Rattus norvegicus | |
0.03 | - |
L-Arg-L-Pro-L-Pro-Gly | - |
Rattus norvegicus | |
0.032 | - |
Arg-Pro-Pro-Gly-Phe-Ser | - |
Rattus norvegicus | |
0.039 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe | - |
Rattus norvegicus | |
0.048 | - |
Arg-Pro-Pro-Gly-Phe | - |
Rattus norvegicus | |
0.051 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro | - |
Rattus norvegicus | |
0.15 | - |
Arg-homoPro-Pro-Ala-NH2 | - |
Rattus norvegicus | |
0.32 | - |
Gly-Pro-Hyp | without Mn2+ | Rattus norvegicus | |
0.35 | - |
Arg-Pro-Pro | without Mn2+ | Rattus norvegicus | |
0.36 | - |
Arg-Pro-Pro | in presence of 4 mM Mn2+ | Rattus norvegicus | |
1 | - |
Arg-Pro-Pro | - |
Rattus norvegicus | |
2 | - |
Gly-Pro-Hyp | in presence of 4 mM Mn2+ | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | membrane-bound | Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 0.3-0.4 mM, 4fold stimulation with Gly-Pro-Hyp as substrate | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, SDS-PAGE | Rattus norvegicus |
220000 | - |
value depends on assay conditions, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | the enzyme contains at least 17% N-linked carbohydrate | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allostatin 1 + H2O | - |
Rattus norvegicus | Ala + ? | cleavage of the Ala1-Pro2 bond | ? | |
Arg-homoPro-Pro-Ala-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? | |
Arg-Pro-Pro + H2O | - |
Rattus norvegicus | Arg + Pro-Pro | - |
? | |
Arg-Pro-Pro-Gly-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser + H2O | - |
Rattus norvegicus | ? | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O | - |
Rattus norvegicus | ? | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | - |
Rattus norvegicus | Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
? | |
Gly-Pro-Hyp + H2O | - |
Rattus norvegicus | Gly + Pro-Hyp | - |
? | |
L-Arg-L-Pro-L-Pro-Gly + H2O | - |
Rattus norvegicus | L-Arg + L-Pro-L-Pro-Gly | - |
? | |
additional information | no cleavage of human erythropoietin | Rattus norvegicus | ? | - |
? | |
neuropeptide Y + H2O | - |
Rattus norvegicus | Tyr + ? | cleavage of the Try1-Pro2 bond | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 6.8, 30 min, 0.1 M potassium phosphate buffer, stable up to | Rattus norvegicus |
70 | 75 | pH 6.8, 30 min, complete loss of activity | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
Arg-Pro-Pro-Gly-Phe-Ser | - |
Rattus norvegicus | |
12 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
Rattus norvegicus | |
13 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe | - |
Rattus norvegicus | |
15 | - |
Arg-Pro-Pro-Gly-Phe | - |
Rattus norvegicus | |
16 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro | - |
Rattus norvegicus | |
17 | - |
L-Arg-L-Pro-L-Pro-Gly | - |
Rattus norvegicus | |
48 | - |
Gly-Pro-Hyp | - |
Rattus norvegicus | |
69 | - |
Gly-Pro-Hyp | in presence of 4 mM Mn2+ | Rattus norvegicus | |
150 | - |
Arg-Pro-Pro | - |
Rattus norvegicus | |
180 | - |
Arg-Pro-Pro | in presence of 4 mM Mn2+ | Rattus norvegicus | |
230 | - |
Arg-Pro-Pro | without 4 mM Mn2+ | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.5 | hydrolysis of Arg-Pro-Pro | Rattus norvegicus |
7 | - |
hydrolysis of bradykinin(1-8), 0.5 mM, in both 0.1 M Hepes buffer or 0.1 M potassium phosphate buffer | Rattus norvegicus |
8 | - |
6 mM imidazole buffer, Arg-Pro-Pro as substrate | Rattus norvegicus |
9 | - |
11 mM imidazole buffer, hydrolysis of Arg-Pro-Pro | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | polyethylene glycol 20000 improves stability in the pH-range 4.5-7.0 | Rattus norvegicus |
7 | 10.5 | optimal stability | Rattus norvegicus |
8.5 | 10 | at 37°C, 2 h, most stable at | Rattus norvegicus |