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Literature summary for 3.4.11.7 extracted from

  • Stressler, T.; Ewert, J.; Merz, M.; Funk, J.; Claassen, W.; Lutz-Wahl, S.; Schmidt, H.; Kuhn, A.; Fischer, L.
    A novel glutamyl (aspartyl)-specific aminopeptidase A from Lactobacillus delbrueckii with promising properties for application (2016), PLoS ONE, 11, e0152139 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.7

Activating Compound

Activating Compound Comment Organism Structure
E64 slight activation Lactobacillus delbrueckii subsp. lactis

Cloned(Commentary)

Cloned (Comment) Organism
gene pepA, recombinant expression of C-terminally His6-tagged enzyme and of untagged enzyme in Escherichia coli strain BL21(DE3) Lactobacillus delbrueckii subsp. lactis
gene pepA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Lactococcus lactis ssp. lactis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 21% at 1.0 mM, complete inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
1,10-phenanthroline complete inhibition at 1-10 mM Lactococcus lactis ssp. lactis
2-mercaptoethanol 58% inhibition at 0.01 mM, 96% at 0.1 mM Lactococcus lactis ssp. lactis
acetone 21.5% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
acetone 37.2% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
dimethylformamide 78.1% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
dimethylformamide 84.9% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
DMSO 15.1% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
DMSO 13.9% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
DTT 12% inhibition at 0.1 mM Lactobacillus delbrueckii subsp. lactis
DTT 69% inhibition at 0.001 mM, 94% at 0.1 mM Lactococcus lactis ssp. lactis
E64 slight inhibition Lactococcus lactis ssp. lactis
EDTA complete inhibition at 1.0 mM Lactobacillus delbrueckii subsp. lactis
EDTA complete inhibition at 1.0 mM Lactococcus lactis ssp. lactis
ethanol 16.3% inhibition at 4.2% v/v Lactobacillus delbrueckii subsp. lactis
ethanol 25.7% inhibition at 4.2% v/v Lactococcus lactis ssp. lactis
imidazole 34% inhibition at 40 mM Lactobacillus delbrueckii subsp. lactis
imidazole 21% inhibition at 40 mM Lactococcus lactis ssp. lactis
L-Asp 39.5% inhibition at 10 mM, no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 33.8% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactobacillus delbrueckii subsp. lactis
L-Asp 21.8% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 24.0% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactococcus lactis ssp. lactis
L-Glu product inhibition of enzyme Lc-PepA, 8.4% inhibition at 0.1 mM, 17.7% at 10 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 1.47% inhibition at 0.1 mM, 31.4% at 10 mM, product inhibition versus substrate L-Glu-4-nitroanilide Lactococcus lactis ssp. lactis
additional information enzyme Lb-PepA is not product-inhibited by L-Glu. Lb-PepA activity decreases significantly with metal salt concentrations above the optimum concentrations determined. Poor inhibition at 1.0 mM 2-mercaptoethanol, no inhibition by pepstatin A at 0.1-10 mM Lactobacillus delbrueckii subsp. lactis
pepstatin A slight inhibition Lactococcus lactis ssp. lactis
PMSF 35% inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
PMSF 32% inhibition at 10 mM Lactococcus lactis ssp. lactis
SDS 73% at 1.0 mM, complete inhibition at 10 mM Lactobacillus delbrueckii subsp. lactis
SDS 63% at 1.0 mM, 97% inhibition at 10 mM Lactococcus lactis ssp. lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Lactococcus lactis ssp. lactis
additional information
-
 additional information Michaelis-Menten kinetics Lactobacillus delbrueckii subsp. lactis
0.19
-
 L-Glu-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactococcus lactis ssp. lactis
0.34
-
 L-Asp-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactococcus lactis ssp. lactis
1.21
-
 L-Asp-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactobacillus delbrueckii subsp. lactis
25.2
-
 L-Glu-4-nitroanilide pH 6.0, 60°C, recombinant enzyme Lactobacillus delbrueckii subsp. lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, best at 0.3125 mM Lactococcus lactis ssp. lactis
Co2+ activates, best at 0.625 mM Lactobacillus delbrueckii subsp. lactis
Mn2+ activates, best at 0.3125 mM Lactococcus lactis ssp. lactis
Mn2+ activates, best at 1.25 mM Lactobacillus delbrueckii subsp. lactis
additional information Lb-PepA activity decreases significantly with metal salt concentrations above the optimum concentrations determined Lactobacillus delbrueckii subsp. lactis
additional information Lc-PepA activity decreases only slightly or is almost constant with metal salt concentrations above the optimum concentrations determined Lactococcus lactis ssp. lactis
Zn2+ activates, best at 5 mM Lactococcus lactis ssp. lactis
Zn2+ slight activation, best at 0.625 mM Lactobacillus delbrueckii subsp. lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
470000
-
 recombinant His6-tagged enzyme, gel filtration Lactococcus lactis ssp. lactis
508000
-
 recombinant His6-tagged enzyme, gel filtration Lactobacillus delbrueckii subsp. lactis

Organism

Organism UniProt Comment Textmining
Lactobacillus delbrueckii subsp. lactis F0HXE4
-
-
Lactobacillus delbrueckii subsp. lactis DSM 20072 F0HXE4
-
-
Lactococcus lactis ssp. lactis
-
-
-
Lactococcus lactis ssp. lactis DSM 20481
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Lactococcus lactis ssp. lactis
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Lactobacillus delbrueckii subsp. lactis

Source Tissue

Source Tissue Comment Organism Textmining
additional information the Lb-PepA activity decreases during the stationary growth phase, which is caused by degradation of the enzyme Lactobacillus delbrueckii subsp. lactis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis L-Asp + 4-nitroaniline
-
 ?
L-Asp-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis L-Asp + 4-nitroaniline
-
 ?
L-Asp-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis DSM 20072 L-Asp + 4-nitroaniline
-
 ?
L-Asp-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis DSM 20481 L-Asp + 4-nitroaniline
-
 ?
L-Glu-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis L-Glu + 4-nitroaniline
-
 ?
L-Glu-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis L-Glu + 4-nitroaniline
-
 ?
L-Glu-4-nitroanilide + H2O
-
 Lactobacillus delbrueckii subsp. lactis DSM 20072 L-Glu + 4-nitroaniline
-
 ?
L-Glu-4-nitroanilide + H2O
-
 Lactococcus lactis ssp. lactis DSM 20481 L-Glu + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
homododecamer 12 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, 12 * 39400, about, sequence calculation Lactococcus lactis ssp. lactis
homododecamer 12 * 41000, recombinant His6-tagged enzyme, SDS-PAGE, 12 * 41100, about, sequence calculation Lactobacillus delbrueckii subsp. lactis

Synonyms

Synonyms Comment Organism
glutamyl (aspartyl)-specific aminopeptidase A
-
 Lactococcus lactis ssp. lactis
glutamyl (aspartyl)-specific aminopeptidase A
-
 Lactobacillus delbrueckii subsp. lactis
Lb-PepA
-
 Lactobacillus delbrueckii subsp. lactis
Lc-PepA
-
 Lactococcus lactis ssp. lactis
More see also EC 3.4.11.21 Lactococcus lactis ssp. lactis
More see also EC 3.4.11.21 Lactobacillus delbrueckii subsp. lactis
PepA
-
 Lactococcus lactis ssp. lactis
PepA
-
 Lactobacillus delbrueckii subsp. lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Lactobacillus delbrueckii subsp. lactis
65
-
-
 Lactococcus lactis ssp. lactis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80 recombinant enzyme, activity range, profile overview Lactococcus lactis ssp. lactis
20 75 recombinant enzyme, activity range, profile overview Lactobacillus delbrueckii subsp. lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 purified recombinant His-tagged enzyme, completely stable for over 70 h Lactococcus lactis ssp. lactis
-
-
 purified recombinant His-tagged enzyme, over 80% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
40
-
 purified recombinant His-tagged enzyme, over 60% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
45
-
 purified recombinant His-tagged enzyme, half-life at 5°C is about 20 h, inactivation within 70 h Lactococcus lactis ssp. lactis
50
-
 purified recombinant His-tagged enzyme, over 15% activity remaining after 70 h Lactobacillus delbrueckii subsp. lactis
55
-
 purified recombinant His-tagged enzyme, half-life at 65°C is about 4-5 h, inactivation within 65 h Lactococcus lactis ssp. lactis
60
-
 purified recombinant His-tagged enzyme, inactivation within 10 h Lactobacillus delbrueckii subsp. lactis
65
-
 purified recombinant His-tagged enzyme, half-life at 65°C is about 2 h, inactivation within 10 h Lactococcus lactis ssp. lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Lactobacillus delbrueckii subsp. lactis
8
-
-
 Lactococcus lactis ssp. lactis

pH Range

pH Minimum pH Maximum Comment Organism
5 10 recombinant enzyme, activity range, profile overview Lactococcus lactis ssp. lactis
5 8 recombinant enzyme, activity range, profile overview Lactobacillus delbrueckii subsp. lactis