Cloned (Comment) | Organism |
---|---|
expression in CHO cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D227A | mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking. Mutant enzyme shows 0.18% of the wild-type activity | Mus musculus |
D227R | mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking | Mus musculus |
R220A | mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking. Mutant enzyme shows 0.3% of the wild-type activity | Mus musculus |
R220D | mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking | Mus musculus |
R220D/D227R | mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamate phosphonate | 0.001 mM, activity of wild-type enzyme is completely abolished | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P16406 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |