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Literature summary for 3.4.11.6 extracted from

  • Cadel, S.; Darmon, C.; Pernier, J.; Herve, G.; Foulon, T.
    The M1 family of vertebrate aminopeptidases: Role of evolutionarily conserved tyrosines in the enzymatic mechanism of aminopeptidase B (2015), Biochimie, 109, 67-77.
    View publication on PubMed
Search for more literature for 3.4.11.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
Y229H Km and kcat (L-Arg-2-naphthylamide) decreased compared to wild-type Rattus norvegicus
Y409F Km and kcat (L-Arg-2-naphthylamide) decreased compared to wild-type Rattus norvegicus
Y441F Km and kcat (L-Arg-2-naphthylamide) decreased compared to wild-type Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
arphamenine A
-
 Rattus norvegicus
Arphamenine B
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0453
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y441F Rattus norvegicus
0.06
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y229H Rattus norvegicus
0.0987
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y409F Rattus norvegicus
0.162
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, wild-type Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Leu-enkephalin + H2O
-
 Rattus norvegicus Arg + Leu-enkephalin
-
 ?
L-Arg-2-naphthylamide + H2O
-
 Rattus norvegicus L-Arg + 2-naphthylamine
-
 ?

Synonyms

Synonyms Comment Organism
aminopeptidase B
-
 Rattus norvegicus
Ap-B
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y441F Rattus norvegicus
4.8
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y409F Rattus norvegicus
9.4
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y229H Rattus norvegicus
25
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, wild-type Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000076
-
 arphamenine A pH 7.4, 37°C, mutant Y229H Rattus norvegicus
0.0000085
-
 arphamenine A pH 7.4, 37°C, wild-type Rattus norvegicus
0.0000085
-
 Arphamenine B pH 7.4, 37°C, mutant Y229H Rattus norvegicus
0.0000103
-
 Arphamenine B pH 7.4, 37°C, wild-type Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
48
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y409F Rattus norvegicus
51
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y441F Rattus norvegicus
154
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, wild-type Rattus norvegicus
157
-
 L-Arg-2-naphthylamide pH 7.4, 37°C, mutant Y229H Rattus norvegicus