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Literature summary for 3.4.11.6 extracted from
- Immobilization of goat brain aminopeptidase B in calcium alginate beads (2009), Biocatal. Biotransform., 27, 96-101.
No PubMed abstract available
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of enzyme with 1% CaCl2 and 2.5% alginate, increase of in stability for immobilized enzyme | Capra aegagrus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
N-naphthalen-2-yl-L-argininamide | free enzyme | Capra aegagrus |  |
| 0.2 | - |
N-naphthalen-2-yl-L-argininamide | immobilized enzyme | Capra aegagrus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97400 | - |
SDS-PAGE | Capra aegagrus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | immobilized enzyme stable at higher concentrations compared to free enzyme | Capra aegagrus |
| Ethanol | 15%, immobilized enzyme still 58% remaining activity, free enzyme inactivated | Capra aegagrus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Capra aegagrus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 279.5fold after heat inactivation, ammonium sulfate fractionation and column chromatographies | Capra aegagrus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Capra aegagrus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.06708 | - |
after purification | Capra aegagrus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine-2-naphthylamide + H2O | assay at 37°C | Capra aegagrus | 2-naphthylamine + L-Arg | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase B | - |
Capra aegagrus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
free enzyme | Capra aegagrus |
| 50 | - |
immobilized enzyme | Capra aegagrus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
immobilized enzyme with 55% remaining enzyme activity at 60°C, free enzyme with 45% remaining activity at 60°C | Capra aegagrus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
free enzyme | Capra aegagrus |
| 7.8 | - |
immobilized enzyme | Capra aegagrus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | free enzyme | Capra aegagrus |
| 6 | 9.5 | immobilized enzyme | Capra aegagrus |
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Release 2023.1 (January 2023)
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