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Literature summary for 3.4.11.5 extracted from

  • Nandan, A.S.; Nampoothiri, K.M.
    Unveiling aminopeptidase P from Streptomyces lavendulae: molecular cloning, expression and biochemical characterization (2014), Enzyme Microb. Technol., 55, 7-13.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Streptomyces lavendulae

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Streptomyces lavendulae
DTT
-
Streptomyces lavendulae
EDTA
-
Streptomyces lavendulae
PMSF
-
Streptomyces lavendulae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enhances activity Streptomyces lavendulae
Cu2+ enhances activity Streptomyces lavendulae
Mg2+ enhances activity Streptomyces lavendulae
Mn2+ enhances activity, atomic absorption studies reveal the presence of Mn2+ in the protein as a co-factor Streptomyces lavendulae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Streptomyces lavendulae
240000
-
native PAGE Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bradykinin + H2O Streptomyces lavendulae
-
?
-
?
Gly-Pro-4-nitroanilide + H2O Streptomyces lavendulae
-
?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae M4MBM1
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Streptomyces lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bradykinin + H2O
-
Streptomyces lavendulae ?
-
?
Gly-Pro-4-nitroanilide + H2O
-
Streptomyces lavendulae ?
-
?

Subunits

Subunits Comment Organism
tetramer 60000 * 4 Streptomyces lavendulae

Synonyms

Synonyms Comment Organism
aminopeptidase P
-
Streptomyces lavendulae
APP
-
Streptomyces lavendulae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
Streptomyces lavendulae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
enzyme retains 50% of activity after 30 min of incubation Streptomyces lavendulae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Streptomyces lavendulae