Cloned (Comment) | Organism |
---|---|
PAP is overexpressed as a His-tag fusion protein under a taka-amylase gene (amyB) promoter with a limited expressing condition in Aspergillus oryzae | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM ZnCl2 and 5 mM beta-mercapthoethanol, respectively, relative activity: 61% | Aspergillus oryzae | |
bestatin | 0.002 mM, relative activity: 65% | Aspergillus oryzae | |
EDTA | 1 mM, relative activity: 68% | Aspergillus oryzae | |
leupeptin | 0.04 mM, relative activity: 68% | Aspergillus oryzae | |
N-tosyl-L-lysine chloromethyl ketone | 4 mM, relative activity: 37% | Aspergillus oryzae | |
N-tosyl-L-phenylalanyl chloromethyl ketone | 0.02 mM, relative activity: 69% | Aspergillus oryzae | |
p-chloromercuribenzenesulfonate | 0.1 mM, relative activity: 0% | Aspergillus oryzae | |
ZnCl2 | 1 mM ZnCl2 + and 5 mM beta-mercapthoethanol, respectively, relative activity: 61% | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
L-Pro-4-nitroanilide | pH 7.5, 30°C | Aspergillus oryzae | |
0.31 | - |
Pro-Ala | pH 7.5, 30°C | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
SDS-PAGE analysis reveals two protein bands (51 kDa and 48 kDa) | Aspergillus oryzae |
51000 | - |
SDS-PAGE analysis reveals two protein bands (51 kDa and 48 kDa) | Aspergillus oryzae |
313000 | - |
gel filtration | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | C6L823 | - |
- |
Purification (Comment) | Organism |
---|---|
enzyme is partially purified through two-step column chromatography using a Ni-IMAC column and a MonoQ column | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
PAP activity in the mycelia grown in rich medium containing glucose (repressing condition) is twice that in starch (inducing condition) | Aspergillus oryzae |
2.4 | - |
substrate: PLSRTLSVAAKK | Aspergillus oryzae |
4.5 | - |
substrate: PPGFSPFR | Aspergillus oryzae |
18.3 | - |
substrate: L-Pro-4-nitroanilide | Aspergillus oryzae |
20.3 | - |
substrate: Pro-2-naphthylamide | Aspergillus oryzae |
22.2 | - |
substrate: Pro-Leu-Gly | Aspergillus oryzae |
25.7 | - |
substrate: Pro-Ala | Aspergillus oryzae |
42.4 | - |
substrate: L-hydroxyproline-2-naphthylamide | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-hydroxyproline-2-naphthylamide + H2O | - |
Aspergillus oryzae | L-hydroxyproline + 2-naphthylamine | - |
? | |
L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae | L-Pro + 4-nitroaniline | - |
? | |
PLSRTLSVAAKK + H2O | - |
Aspergillus oryzae | Pro + LSRTLSVAAKK | - |
? | |
PPGFSPFR + H2O | - |
Aspergillus oryzae | Pro + PGFSPFR | - |
? | |
Pro-2-naphthylamide + H2O | - |
Aspergillus oryzae | Pro + 2-naphthylamide | - |
? | |
Pro-Ala + H2O | PamA releases Pro from amino terminus of a dipeptide Pro-Ala, and not from Ala-Pro | Aspergillus oryzae | Pro + Ala | - |
? | |
Pro-Leu-Gly + H2O | - |
Aspergillus oryzae | Leu-Gly + Pro | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 48000, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
pamA | - |
Aspergillus oryzae |
PAP | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme gets inactivated above 50°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at and optimal pH | Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | - |
Aspergillus oryzae |