Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli | Serratia marcescens |
Protein Variants | Comment | Organism |
---|---|---|
C271A | site-directed mutagenesis, sensitive to inhibition by 4-chloromercuribenzoic acid | Serratia marcescens |
C74A | site-directed mutagenesis, sensitive to inhibition by 4-chloromercuribenzoic acid | Serratia marcescens |
C74A/C271A | site-directed mutagenesis, not sensitive to inhibition by 4-chloromercuribenzoic acid | Serratia marcescens |
E204Q | site-directed mutagenesis, 4% of the wild-type catalytic efficiency | Serratia marcescens |
F139A | site-directed mutagenesis, 80fold decreased catalytic activity compared to the wild-type enzyme | Serratia marcescens |
R136A | site-directed mutagenesis, shows decreased activity compared to the wild-type enzyme, but retains arylamidase activity | Serratia marcescens |
Y149A | site-directed mutagenesis, unaltered catalytic efficiency compared to the wild-type enzyme | Serratia marcescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoic acid | - |
Serratia marcescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
proline beta-naphthylamide | pH 8.0, 37°C, mutant Y149A | Serratia marcescens | |
0.42 | - |
proline beta-naphthylamide | pH 8.0, 37°C, wild-type enzyme | Serratia marcescens | |
0.46 | - |
proline beta-naphthylamide | pH 8.0, 37°C, mutant R136A | Serratia marcescens | |
0.82 | - |
proline beta-naphthylamide | pH 8.0, 37°C, mutant E204Q | Serratia marcescens | |
1.38 | - |
proline beta-naphthylamide | pH 8.0, 37°C, mutant F139A | Serratia marcescens | |
2.07 | - |
L-Pro-L-Ala | pH 8.0, 37°C, wild-type enzyme | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Serratia marcescens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of N-terminal proline from a peptide | substrate recognition mechanism, Phe139 and Glu204 recognize the pyrrolidine ring and the amino group of proline at the S1 site, Arg136 recognizes the P'1 site | Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Pro-L-Ala + H2O | no activity of mutant R136A | Serratia marcescens | L-Pro + L-Ala | - |
? | |
additional information | substrate specificity and binding mechanism | Serratia marcescens | ? | - |
? | |
proline beta-naphthylamide + H2O | - |
Serratia marcescens | proline + beta-naphthylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme structure, active site structure | Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Serratia marcescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | 1.97 | proline beta-naphthylamide | pH 8.0, 37°C, mutant R136A | Serratia marcescens | |
0.052 | 2.1 | proline beta-naphthylamide | pH 8.0, 37°C, wild-type enzyme | Serratia marcescens | |
0.08 | - |
proline beta-naphthylamide | pH 8.0, 37°C, mutant E204Q | Serratia marcescens | |
0.41 | - |
proline beta-naphthylamide | pH 8.0, 37°C, mutant F139A | Serratia marcescens | |
1.4 | - |
proline beta-naphthylamide | pH 8.0, 37°C, mutant Y149A | Serratia marcescens | |
8.87 | - |
proline beta-naphthylamide | pH 8.0, 37°C, mutant R136A | Serratia marcescens | |
9.81 | - |
proline beta-naphthylamide | pH 8.0, 37°C, wild-type enzyme | Serratia marcescens | |
16.8 | - |
L-Pro-L-Ala | pH 8.0, 37°C, wild-type enzyme | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Serratia marcescens |