Literature summary for 3.4.11.24 extracted from

Usuki, H.; Uesugi, Y.; Arima, J.; Yamamoto, Y.; Iwabuchi, M.; Hatanaka, T.
Engineered transaminopeptidase, aminolysin-S for catalysis of peptide bond formation to give linear and cyclic dipeptides by one-pot reaction (2010), Chem. Commun. (Camb. ), 46, 580-582.

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Protein Variants

Protein Variants	Comment	Organism
S502C	mutant without aminopeptidase activity but with peptide synthesis activity	Streptomyces thermocyaneoviolaceus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces thermocyaneoviolaceus	-	-	-
Streptomyces thermocyaneoviolaceus NBRC14271	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-alanine-4-nitroanilide + H2O	-	Streptomyces thermocyaneoviolaceus	L-Ala + 4-nitroaniline	-	?
L-alanine-4-nitroanilide + H2O	-	Streptomyces thermocyaneoviolaceus NBRC14271	L-Ala + 4-nitroaniline	-	?
L-phenylalanine ethyl ester + L-phenylalanine + H2O	assay at 50°C	Streptomyces thermocyaneoviolaceus	L-Phe-L-Phe + cyclo(L-phenylalanine-L-phenylalanine) + L-Phe-L-Phe ethyl ester	-	?
L-phenylalanine ethyl ester + L-phenylalanine + H2O	assay at 50°C	Streptomyces thermocyaneoviolaceus NBRC14271	L-Phe-L-Phe + cyclo(L-phenylalanine-L-phenylalanine) + L-Phe-L-Phe ethyl ester	-	?
L-phenylalanine-4-nitroanilide + H2O	-	Streptomyces thermocyaneoviolaceus	L-Phe + 4-nitroaniline	-	?
L-phenylalanine-4-nitroanilide + H2O	-	Streptomyces thermocyaneoviolaceus NBRC14271	L-Phe + 4-nitroaniline	-	?
L-proline-p-nitroanilide + H2O	-	Streptomyces thermocyaneoviolaceus	L-Pro + 4-nitroaniline	-	?
L-proline-p-nitroanilide + H2O	-	Streptomyces thermocyaneoviolaceus NBRC14271	L-Pro + 4-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
aminolysin-S	-	Streptomyces thermocyaneoviolaceus
transaminopeptidase	-	Streptomyces thermocyaneoviolaceus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
95	-	enzyme inactivation after 30 min at 95°C	Streptomyces thermocyaneoviolaceus

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Release 2023.1 (January 2023)