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Literature summary for 3.4.11.24 extracted from

  • Arima, J.; Iwabuchi, M.; Hatanaka, T.
    Gene cloning and overproduction of an aminopeptidase from Streptomyces septatus TH-2, and comparison with a calcium-activated enzyme from Streptomyces griseus (2004), Biochem. Biophys. Res. Commun., 317, 531-538.
    View publication on PubMed
Search for more literature for 3.4.11.24

Cloned(Commentary)

Cloned (Comment) Organism
gene Sgap, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli Streptomyces griseus
gene Ssap, genomic library screening, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli strain BL21(DE3) Streptomyces septatus

Inhibitors

Inhibitors Comment Organism Structure
amastatin
-
 Streptomyces griseus
amastatin
-
 Streptomyces septatus
bestatin
-
 Streptomyces griseus
bestatin
-
 Streptomyces septatus
Ca2+
-
 Streptomyces griseus
Ca2+ slight inhibition at 1 mM Streptomyces septatus
Cd2+
-
 Streptomyces griseus
Cd2+
-
 Streptomyces septatus
Cu2+
-
 Streptomyces griseus
Cu2+
-
 Streptomyces septatus
Fe2+
-
 Streptomyces griseus
Fe2+
-
 Streptomyces septatus
L-leucine very low product inhibition Streptomyces griseus
L-leucine
-
 Streptomyces septatus
Mg2+
-
 Streptomyces griseus
Mg2+ slight inhibition at 0.1-1 mM Streptomyces septatus
Mn2+
-
 Streptomyces griseus
Mn2+
-
 Streptomyces septatus
additional information very low product inhibition by L-leucine Streptomyces septatus
Ni2+
-
 Streptomyces griseus
Ni2+
-
 Streptomyces septatus
Zn2+
-
 Streptomyces griseus
Zn2+
-
 Streptomyces septatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ Streptomyces septatus
0.34
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ Streptomyces septatus
0.65
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ Streptomyces griseus
2.3
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ Streptomyces griseus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates the enzyme with some substrates, about 3fold with L-Leu-4-nitroanilide, and affects substrate specificity, e.g. decreases the activity with Lys-4-nitroanilide Streptomyces griseus
Co2+ slightly activating at 0.1-1 mM Streptomyces griseus
additional information Ca2+ and Co2+ do not affect the enzyme's substrate specificity at 0.1-1 mM Streptomyces septatus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-
Streptomyces septatus Q75V72
-
-
Streptomyces septatus TH-2 Q75V72
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ion exchange chromatography, ammonium sulfate fractionation, and gel filtration, recombinant enzyme from Escherichia coli strain BL21(DE3) culture supernatant by ion exchange chromatography Streptomyces septatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O very low activity Streptomyces septatus L-Ala + 4-nitroaniline
-
 ?
L-Ala-4-nitroanilide + H2O very low activity Streptomyces septatus TH-2 L-Ala + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O low activity Streptomyces septatus L-Arg + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O low activity Streptomyces septatus TH-2 L-Arg + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O best substrate Streptomyces griseus L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O best substrate Streptomyces septatus L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O best substrate Streptomyces septatus TH-2 L-Leu + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O low activity Streptomyces griseus L-Lys + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O low activity Streptomyces septatus L-Lys + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O low activity Streptomyces septatus TH-2 L-Lys + 4-nitroaniline
-
 ?
L-Met-4-nitroanilide + H2O low activity Streptomyces griseus L-Met + 4-nitroaniline
-
 ?
L-Met-4-nitroanilide + H2O low activity Streptomyces septatus L-Met + 4-nitroaniline
-
 ?
L-Phe-4-nitroanilide + H2O low activity Streptomyces griseus L-Phe + 4-nitroaniline
-
 ?
L-Phe-4-nitroanilide + H2O low activity Streptomyces septatus L-Phe + 4-nitroaniline
-
 ?
L-Pro-4-nitroanilide + H2O very low activity Streptomyces septatus L-Pro + 4-nitroaniline
-
 ?
additional information no activity with L-Glu-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide Streptomyces septatus ?
-
 ?
additional information no activity with L-Glu-4-nitroanilide, L-Arg-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide, L-Ala-4-nitroanilide and L-Pro-4-nitroanilide are poor substrates Streptomyces griseus ?
-
 ?
additional information no activity with L-Glu-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide Streptomyces septatus TH-2 ?
-
 ?

Synonyms

Synonyms Comment Organism
SGAP
-
 Streptomyces griseus
SSAP
-
 Streptomyces septatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 in absence of Ca2+ Streptomyces griseus
75
-
-
 Streptomyces septatus
75
-
 in presence of Ca2+ Streptomyces griseus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75 in absence of Ca2+ Streptomyces griseus
30 90
-
 Streptomyces septatus
30 90 in presence of Ca2+ Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Streptomyces griseus
additional information
-
-
 Streptomyces septatus
40 60 30 min, recombinant enzyme, stable Streptomyces septatus
70
-
 30 min, 50% loss of activity, in absence of Ca2+ Streptomyces griseus
78
-
 30 min, recombinant enzyme, 50% loss of activity Streptomyces septatus
80
-
 30 min, 50% loss of activity, in presence of Ca2+ Streptomyces griseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41.5
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ Streptomyces septatus
42.1
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ Streptomyces septatus
75.3
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ Streptomyces griseus
223
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Streptomyces griseus
8.5
-
-
 Streptomyces septatus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH profile Streptomyces griseus
additional information
-
 pH profile Streptomyces septatus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000011
-
 amastatin pH 8.0, 37°C, recombinant enzyme Streptomyces griseus
0.00079
-
 amastatin pH 8.0, 37°C, recombinant enzyme Streptomyces septatus
0.0039
-
 bestatin pH 8.0, 37°C, recombinant enzyme Streptomyces griseus
0.065
-
 bestatin pH 8.0, 37°C, recombinant enzyme Streptomyces septatus
29
-
 L-leucine above, pH 8.0, 37°C, recombinant enzyme Streptomyces griseus
100
-
 L-leucine above, pH 8.0, 37°C, recombinant enzyme Streptomyces septatus