Cloned (Comment) | Organism |
---|---|
gene Sgap, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli | Streptomyces griseus |
gene Ssap, genomic library screening, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli strain BL21(DE3) | Streptomyces septatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amastatin | - |
Streptomyces griseus | |
amastatin | - |
Streptomyces septatus | |
bestatin | - |
Streptomyces griseus | |
bestatin | - |
Streptomyces septatus | |
Ca2+ | - |
Streptomyces griseus | |
Ca2+ | slight inhibition at 1 mM | Streptomyces septatus | |
Cd2+ | - |
Streptomyces griseus | |
Cd2+ | - |
Streptomyces septatus | |
Cu2+ | - |
Streptomyces griseus | |
Cu2+ | - |
Streptomyces septatus | |
Fe2+ | - |
Streptomyces griseus | |
Fe2+ | - |
Streptomyces septatus | |
L-leucine | very low product inhibition | Streptomyces griseus | |
L-leucine | - |
Streptomyces septatus | |
Mg2+ | - |
Streptomyces griseus | |
Mg2+ | slight inhibition at 0.1-1 mM | Streptomyces septatus | |
Mn2+ | - |
Streptomyces griseus | |
Mn2+ | - |
Streptomyces septatus | |
additional information | very low product inhibition by L-leucine | Streptomyces septatus | |
Ni2+ | - |
Streptomyces griseus | |
Ni2+ | - |
Streptomyces septatus | |
Zn2+ | - |
Streptomyces griseus | |
Zn2+ | - |
Streptomyces septatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ | Streptomyces septatus | |
0.34 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ | Streptomyces septatus | |
0.65 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ | Streptomyces griseus | |
2.3 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ | Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates the enzyme with some substrates, about 3fold with L-Leu-4-nitroanilide, and affects substrate specificity, e.g. decreases the activity with Lys-4-nitroanilide | Streptomyces griseus | |
Co2+ | slightly activating at 0.1-1 mM | Streptomyces griseus | |
additional information | Ca2+ and Co2+ do not affect the enzyme's substrate specificity at 0.1-1 mM | Streptomyces septatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Streptomyces septatus | Q75V72 | - |
- |
Streptomyces septatus TH-2 | Q75V72 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by ion exchange chromatography, ammonium sulfate fractionation, and gel filtration, recombinant enzyme from Escherichia coli strain BL21(DE3) culture supernatant by ion exchange chromatography | Streptomyces septatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-4-nitroanilide + H2O | very low activity | Streptomyces septatus | L-Ala + 4-nitroaniline | - |
? | |
L-Ala-4-nitroanilide + H2O | very low activity | Streptomyces septatus TH-2 | L-Ala + 4-nitroaniline | - |
? | |
L-Arg-4-nitroanilide + H2O | low activity | Streptomyces septatus | L-Arg + 4-nitroaniline | - |
? | |
L-Arg-4-nitroanilide + H2O | low activity | Streptomyces septatus TH-2 | L-Arg + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | best substrate | Streptomyces griseus | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | best substrate | Streptomyces septatus | L-Leu + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | best substrate | Streptomyces septatus TH-2 | L-Leu + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | low activity | Streptomyces griseus | L-Lys + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | low activity | Streptomyces septatus | L-Lys + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | low activity | Streptomyces septatus TH-2 | L-Lys + 4-nitroaniline | - |
? | |
L-Met-4-nitroanilide + H2O | low activity | Streptomyces griseus | L-Met + 4-nitroaniline | - |
? | |
L-Met-4-nitroanilide + H2O | low activity | Streptomyces septatus | L-Met + 4-nitroaniline | - |
? | |
L-Phe-4-nitroanilide + H2O | low activity | Streptomyces griseus | L-Phe + 4-nitroaniline | - |
? | |
L-Phe-4-nitroanilide + H2O | low activity | Streptomyces septatus | L-Phe + 4-nitroaniline | - |
? | |
L-Pro-4-nitroanilide + H2O | very low activity | Streptomyces septatus | L-Pro + 4-nitroaniline | - |
? | |
additional information | no activity with L-Glu-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide | Streptomyces septatus | ? | - |
? | |
additional information | no activity with L-Glu-4-nitroanilide, L-Arg-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide, L-Ala-4-nitroanilide and L-Pro-4-nitroanilide are poor substrates | Streptomyces griseus | ? | - |
? | |
additional information | no activity with L-Glu-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide | Streptomyces septatus TH-2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SGAP | - |
Streptomyces griseus |
SSAP | - |
Streptomyces septatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
in absence of Ca2+ | Streptomyces griseus |
75 | - |
- |
Streptomyces septatus |
75 | - |
in presence of Ca2+ | Streptomyces griseus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 75 | in absence of Ca2+ | Streptomyces griseus |
30 | 90 | - |
Streptomyces septatus |
30 | 90 | in presence of Ca2+ | Streptomyces griseus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces griseus |
additional information | - |
- |
Streptomyces septatus |
40 | 60 | 30 min, recombinant enzyme, stable | Streptomyces septatus |
70 | - |
30 min, 50% loss of activity, in absence of Ca2+ | Streptomyces griseus |
78 | - |
30 min, recombinant enzyme, 50% loss of activity | Streptomyces septatus |
80 | - |
30 min, 50% loss of activity, in presence of Ca2+ | Streptomyces griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41.5 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ | Streptomyces septatus | |
42.1 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ | Streptomyces septatus | |
75.3 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ | Streptomyces griseus | |
223 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptomyces griseus |
8.5 | - |
- |
Streptomyces septatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile | Streptomyces griseus |
additional information | - |
pH profile | Streptomyces septatus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000011 | - |
amastatin | pH 8.0, 37°C, recombinant enzyme | Streptomyces griseus | |
0.00079 | - |
amastatin | pH 8.0, 37°C, recombinant enzyme | Streptomyces septatus | |
0.0039 | - |
bestatin | pH 8.0, 37°C, recombinant enzyme | Streptomyces griseus | |
0.065 | - |
bestatin | pH 8.0, 37°C, recombinant enzyme | Streptomyces septatus | |
29 | - |
L-leucine | above, pH 8.0, 37°C, recombinant enzyme | Streptomyces griseus | |
100 | - |
L-leucine | above, pH 8.0, 37°C, recombinant enzyme | Streptomyces septatus |