Literature summary for 3.4.11.24 extracted from

Gilboa, R.; Spungin-Bialik, A.; Wohlfahrt, G.; Schomburg, D.; Blumberg, S.; Shoham, G.
Interactions of Streptomyces griseus aminopeptidase with amino acid reaction products and their implications toward a catalytic mechanism (2001), Proteins, 44, 490-504.

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Application

Application	Comment	Organism
biotechnology	enzyme is attractive for diverse applications, e.g. the processing of recombinant DNA proteins and fusion protein production due to its heat stability, high activity, and small size	Streptomyces griseus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme complexed with L-methionine, L-phenylalanine, or L-leucine, hanging-drop vapour diffusion method, protein solution: 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, 100 mM L-methionine or 200 mM L-leucine, plus equal volume of precipitant solution: 24% w/v PEG 4000, 0.1 M ammonium sulfate, equilibrated against 1 ml of reservoir precipitant solution, 3-4 days, cyrstals of enzyme complexed with L-Phe were precipitated with 0.1 M acetate buffer, pH 5.5 instead in the same procedure within 8-10 weeks, X-ray complex structure determination at 1.6 A resolution and analysis	Streptomyces griseus

Crystallization (Comment)

Organism

purified enzyme complexed with L-methionine, L-phenylalanine, or L-leucine, hanging-drop vapour diffusion method, protein solution: 18 mg/ml protein, 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 6 mM CaCl2, 100 mM L-methionine or 200 mM L-leucine, plus equal volume of precipitant solution: 24% w/v PEG 4000, 0.1 M ammonium sulfate, equilibrated against 1 ml of reservoir precipitant solution, 3-4 days, cyrstals of enzyme complexed with L-Phe were precipitated with 0.1 M acetate buffer, pH 5.5 instead in the same procedure within 8-10 weeks, X-ray complex structure determination at 1.6 A resolution and analysis

Streptomyces griseus

Inhibitors

Inhibitors	Comment	Organism
L-leucine	binding mechanism and structure	Streptomyces griseus
L-methionine	binding mechanism and structure	Streptomyces griseus
L-phenylalanine	binding mechanism and structure	Streptomyces griseus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	double-zinc exopeptidase	Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptide + H2O	Streptomyces griseus	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseus	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
release of an N-terminal amino acid with a preference for large hydrophobic amino-terminus residues	catalytic mechanism, high preference towards large hydrophobic amino terminus residues, active site structure, Glu131 is involved in the catalytic mechanism, enzyme-substrate and enzyme-product complex modeling	Streptomyces griseus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
peptide + H2O	-	Streptomyces griseus	?	-	?
peptide + H2O	high preference towards large hydrophobic amino terminus residues	Streptomyces griseus	?	-	?

Synonyms

Synonyms	Comment	Organism
M28.003	Merops-ID	Streptomyces griseus
SGAP	-	Streptomyces griseus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.4	-	about	Streptomyces griseus