Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Streptomyces griseus | |
additional information | nonchelating 1,7-phenanthroline has little effect on the activity | Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Streptomyces griseus | |
0.53 | - |
L-Val-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
0.58 | - |
L-Leu-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
4.08 | - |
L-Ala-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, binds to the enzyme, activation level with different enzyme substrates, overview | Streptomyces griseus | |
additional information | substitution of the Zn2+ ion by Mn2+ or Co2+ results in altered substrate specificity, e.g. the Co2+ containing enzyme highly prefers L-alanine-4-nitroanilide | Streptomyces griseus | |
Zn2+ | metalloprotease, dependent on | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | Streptomyces griseus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
enzyme purified from commercial product | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus | Streptomyces griseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-4-nitroanilide + H2O | - |
Streptomyces griseus | L-Ala + 4-nitroaniline | - |
? | |
L-alanine-4-nitroanilide + H2O | good substrate | Streptomyces griseus | L-alanine + 4-nitroaniline | - |
? | |
L-leucine-4-nitroanilide + H2O | best of the amino acid 4-nitroanilide substrates | Streptomyces griseus | L-leucine + 4-nitroaniline | - |
? | |
L-lysine-4-nitroanilide + H2O | good substrate | Streptomyces griseus | L-lysine + 4-nitroaniline | - |
? | |
L-methionine-4-nitroanilide + H2O | very good substrate | Streptomyces griseus | L-methionine + 4-nitroaniline | - |
? | |
L-phenylalanine-4-nitroanilide + H2O | very good substrate | Streptomyces griseus | L-phenylalanine + 4-nitroaniline | - |
? | |
L-proline-4-nitroanilide + H2O | good substrate | Streptomyces griseus | L-proline + 4-nitroaniline | - |
? | |
L-valine-4-nitroanilide + H2O | good substrate | Streptomyces griseus | L-valine + 4-nitroaniline | - |
? | |
additional information | no activity with Gly-Pro-4-nitroanilide and Ala-Pro-4-nitroanilide, no acitivity with Xaa-Pro N-terminal sequences, glycine-4-nitroanilide and acidic amino acid 4-nitroanilide are very poor substrates | Streptomyces griseus | ? | - |
? | |
peptide + H2O | - |
Streptomyces griseus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M28.003 | Merops-ID | Streptomyces griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.191 | - |
L-Val-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
1.71 | - |
L-Ala-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
2.94 | - |
L-Ala-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
391 | - |
L-Leu-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces griseus |