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Literature summary for 3.4.11.22 extracted from

  • Nishiwaki, T.; Hayashi, K.
    Purification and characterization of an aminopeptidase from the edible basidiomycete Grifola frondosa (2001), Biosci. Biotechnol. Biochem., 65, 424-427.
    View publication on PubMed
Search for more literature for 3.4.11.22

Application

Application Comment Organism
nutrition the enzyme is useful to reduce bitterness of food products by hydrolysis of peptides with hydrophobic amino acids causing the bitter taste Grifola frondosa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inactivation Grifola frondosa
bestatin strong inhibition Grifola frondosa
Co2+ 66% inhibition at 0.03 mM Grifola frondosa
Cu2+ 32% inhibition at 0.03 mM Grifola frondosa
EDTA inactivation Grifola frondosa
Mg2+ complete inhibition at 0.03 mM Grifola frondosa
Mn2+ 94% inhibition at 0.03 mM Grifola frondosa
additional information no inhibition by DFP Grifola frondosa
Zn2+ 42% inhibition at 0.03 mM Grifola frondosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 L-Val-4-nitroanilide pH 8.5, 65°C Grifola frondosa
0.31
-
 L-Phe-4-nitroanilide pH 8.5, 65°C Grifola frondosa
0.35
-
 L-Leu-4-nitroanilide pH 8.5, 65°C Grifola frondosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is no metallopeptidase Grifola frondosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 gel filtration Grifola frondosa
30000
-
 1 * 30000, SDS-PAGE Grifola frondosa

Organism

Organism UniProt Comment Textmining
Grifola frondosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 178fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, and ion exchange chromatography Grifola frondosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.55
-
 purified enzyme Grifola frondosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O best substrate Grifola frondosa L-Leu + 4-nitroaniline
-
 ?
L-Phe-4-nitroanilide + H2O
-
 Grifola frondosa L-Phe + 4-nitroaniline
-
 ?
L-Val-4-nitroanilide + H2O low activity Grifola frondosa L-Val + 4-nitroaniline
-
 ?
additional information the enzyme prefers peptide substrates with an N-terminal hydrophobic amino acid residue Grifola frondosa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Grifola frondosa

Synonyms

Synonyms Comment Organism
Aminopeptidase
-
 Grifola frondosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Grifola frondosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 60 min, purified enzyme, stable Grifola frondosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 L-Val-4-nitroanilide pH 8.5, 65°C Grifola frondosa
0.95
-
 L-Phe-4-nitroanilide pH 8.5, 65°C Grifola frondosa
1.43
-
 L-Leu-4-nitroanilide pH 8.5, 65°C Grifola frondosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Grifola frondosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10.5 60 min, purified enzyme, stable Grifola frondosa