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Literature summary for 3.4.11.22 extracted from

  • Chien, H.C.R.; Lin, L.L.; Chao, S.H.; Chen, C.C.; Wang, W.C.; Shaw, C.Y.; Tsai, Y.C.; Hu, H.Y.; Hsu, W.H.
    Purification, characterization, and genetic analysis of a leucine aminopeptidase from Aspergillus sojae (2002), Biochim. Biophys. Acta, 1576, 119-126.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli, phylogenetic comparison to other organisms Aspergillus sojae

Inhibitors

Inhibitors Comment Organism Structure
dipicolinic acid 97% inhibition at 5 mM, Mg2+, Zn2+, and Co2+ partly protect Aspergillus sojae
EDTA 76% inhibition at 10 mM, inhibitory effect is increased by addition of Mg2+, and reversed by addition of Zn2+ and Co2+ Aspergillus sojae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus sojae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can substitute for Zn2+ Aspergillus sojae
Zn2+ metalloenzyme Aspergillus sojae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE, x * 41061, amino acid sequence calculation Aspergillus sojae
41061
-
x * 37000, SDS-PAGE, x * 41061, amino acid sequence calculation Aspergillus sojae

Organism

Organism UniProt Comment Textmining
Aspergillus sojae Q8J2N2 strain ATCC 42249, single copy lap gene
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 3% of the molecular weight counts for N-glycosylated carbohydrate, putative glycosylation sites are Asn87 and Asn288 Aspergillus sojae

Purification (Commentary)

Purification (Comment) Organism
further purification of the commercial preparation, to homogeneity Aspergillus sojae

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Aspergillus sojae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-4-nitroanilide + H2O low activity Aspergillus sojae Ala + 4-nitroaniline
-
?
Arg-4-nitroanilide + H2O
-
Aspergillus sojae Arg + 4-nitroaniline
-
?
Glu-4-nitroanilide + H2O low activity Aspergillus sojae Glu + 4-nitroaniline
-
?
Leu-4-nitroanilide + H2O
-
Aspergillus sojae Leu + 4-nitroaniline
-
?
Lys-4-nitroanilide + H2O
-
Aspergillus sojae Lys + 4-nitroaniline
-
?
Phe-4-nitroanilide + H2O
-
Aspergillus sojae Phe + 4-nitroaniline
-
?
Pro-4-nitroanilide + H2O low activity Aspergillus sojae Pro + 4-nitroaniline
-
?
Val-4-nitroanilide + H2O low activity Aspergillus sojae Val + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE, x * 41061, amino acid sequence calculation Aspergillus sojae
More enzyme contains a leader peptide of 77 amino acids including a 18 amino acid signal peptide Aspergillus sojae

Synonyms

Synonyms Comment Organism
LAP
-
Aspergillus sojae
leucine aminopeptidase
-
Aspergillus sojae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Aspergillus sojae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 more than 80% of maximal activity at this range Aspergillus sojae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
below, 3 days, more than 90% remaining activity Aspergillus sojae
50
-
1 h, loss of 50% activity Aspergillus sojae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Aspergillus sojae

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10
-
Aspergillus sojae