Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli, phylogenetic comparison to other organisms | Aspergillus sojae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dipicolinic acid | 97% inhibition at 5 mM, Mg2+, Zn2+, and Co2+ partly protect | Aspergillus sojae | |
EDTA | 76% inhibition at 10 mM, inhibitory effect is increased by addition of Mg2+, and reversed by addition of Zn2+ and Co2+ | Aspergillus sojae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus sojae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can substitute for Zn2+ | Aspergillus sojae | |
Zn2+ | metalloenzyme | Aspergillus sojae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE, x * 41061, amino acid sequence calculation | Aspergillus sojae |
41061 | - |
x * 37000, SDS-PAGE, x * 41061, amino acid sequence calculation | Aspergillus sojae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sojae | Q8J2N2 | strain ATCC 42249, single copy lap gene | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 3% of the molecular weight counts for N-glycosylated carbohydrate, putative glycosylation sites are Asn87 and Asn288 | Aspergillus sojae |
Purification (Comment) | Organism |
---|---|
further purification of the commercial preparation, to homogeneity | Aspergillus sojae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Aspergillus sojae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-4-nitroanilide + H2O | low activity | Aspergillus sojae | Ala + 4-nitroaniline | - |
? | |
Arg-4-nitroanilide + H2O | - |
Aspergillus sojae | Arg + 4-nitroaniline | - |
? | |
Glu-4-nitroanilide + H2O | low activity | Aspergillus sojae | Glu + 4-nitroaniline | - |
? | |
Leu-4-nitroanilide + H2O | - |
Aspergillus sojae | Leu + 4-nitroaniline | - |
? | |
Lys-4-nitroanilide + H2O | - |
Aspergillus sojae | Lys + 4-nitroaniline | - |
? | |
Phe-4-nitroanilide + H2O | - |
Aspergillus sojae | Phe + 4-nitroaniline | - |
? | |
Pro-4-nitroanilide + H2O | low activity | Aspergillus sojae | Pro + 4-nitroaniline | - |
? | |
Val-4-nitroanilide + H2O | low activity | Aspergillus sojae | Val + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE, x * 41061, amino acid sequence calculation | Aspergillus sojae |
More | enzyme contains a leader peptide of 77 amino acids including a 18 amino acid signal peptide | Aspergillus sojae |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Aspergillus sojae |
leucine aminopeptidase | - |
Aspergillus sojae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Aspergillus sojae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | more than 80% of maximal activity at this range | Aspergillus sojae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
below, 3 days, more than 90% remaining activity | Aspergillus sojae |
50 | - |
1 h, loss of 50% activity | Aspergillus sojae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Aspergillus sojae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | - |
Aspergillus sojae |