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Literature summary for 3.4.11.22 extracted from

  • Metz, G.; Röhm.K.H.
    Yeast aminopeptidase I. Chemical composition and catalytic properties (1976), Biochim. Biophys. Acta, 429, 933-949.
    View publication on PubMed
Search for more literature for 3.4.11.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 Ala-Thr-Ala methyl ester
-
 Saccharomyces cerevisiae
3.2
-
 Ala-Thr-Ala
-
 Saccharomyces cerevisiae
6
-
 L-Ala-L-Th
-
 Saccharomyces cerevisiae
7
-
 L-Leu-Gly-L-Leu
-
 Saccharomyces cerevisiae
12
-
 Ala-Gly-Gly
-
 Saccharomyces cerevisiae
14
-
 L-Ala-Gly-Gly ethyl este
-
 Saccharomyces cerevisiae
40
-
 Ala-Gly
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Br- activation Saccharomyces cerevisiae
Zn2+ activation Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 12 * 51000, SDS-PAGE Saccharomyces cerevisiae
610000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 12% w/w neutral hexoses, 0.5% w/w hexosamines, no sialic acids Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
980
-
 substrate Leu-Gly-Gly Saccharomyces cerevisiae
3000
-
 substrate Ala-Thr-Ala Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
frozen, 50 mM ammonium acetate buffer, pH 6.2 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-Gly + H2O
-
 Saccharomyces cerevisiae L-Ala + Gly
-
 ?
L-Ala-Gly-Gly + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
L-Ala-Gly-Gly ethyl ester + H2O
-
 Saccharomyces cerevisiae L-Ala + Gly-Gly ethyl ester
-
 ?
L-Ala-L-Asp-L-Phe methyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
L-Ala-L-Leu + H2O
-
 Saccharomyces cerevisiae L-Ala + L-Leu
-
 ?
L-Ala-L-Thr + H2O
-
 Saccharomyces cerevisiae L-Ala + L-Thr
-
 ?
L-Ala-L-Thr-Gly methyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
L-Ala-L-Thr-L-Ala + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
L-Ala-L-Thr-L-Ala methyl ester + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
L-Leu-Gly + H2O
-
 Saccharomyces cerevisiae L-Leu + Gly
-
 ?
L-Leu-Gly-Gly + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
L-Leu-Gly-L-Leu + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
L-Leu-L-Ala + H2O
-
 Saccharomyces cerevisiae L-Leu + L-Ala
-
 ?
L-Leu-L-Leu + H2O
-
 Saccharomyces cerevisiae L-Leu + L-Leu
-
 ?
L-Phe-Gly + H2O
-
 Saccharomyces cerevisiae L-Phe + Gly
-
 ?
L-Phe-Gly-Gly + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
additional information overview, peptidyl substrates, Leu and other hydrophobic amino acids are best substrates, Gly and other charged amino acids are split off more slowly Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 51000, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33300
-
 Ala-Thr-Ala
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 absence of activators Saccharomyces cerevisiae
7 8.5 presence of Zn2+ and Cl- Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 4°C, 3 weeks, 10% loss of activity Saccharomyces cerevisiae