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Literature summary for 3.4.11.2 extracted from

  • Chen, J.; Aimanova, K.G.; Pan, S.; Gill, S.S.
    Identification and characterization of Aedes aegypti aminopeptidase N as a putative receptor of Bacillus thuringiensis Cry11A toxin (2009), Insect Biochem. Mol. Biol., 39, 688-696.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH10B cells (binds Cry11Aa toxin with high affinity) and in Sf21 cells (enzymatically active, has a GPI-anchor but does not bind Cry11Aa toxin) Aedes aegypti

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Aedes aegypti 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 in Sf12 cells infected with recombinant baculovirus containing full-length APN,1 the anti-APN1 antibody detects a 110000 Da band, gel filtration Aedes aegypti
112000
-
 calculated from amino acid sequence Aedes aegypti
140000
-
 native enzyme, gel filtration Aedes aegypti

Organism

Organism UniProt Comment Textmining
Aedes aegypti Q16L33
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the native but Sf21 cell-expressed APN1 contains N-acetylglucosamine moieties Aedes aegypti

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephadex G25 gel filtration Aedes aegypti

Source Tissue

Source Tissue Comment Organism Textmining
epithelial cell the protein localizes to the apical side of posterior midgut epithelial cells of larva Aedes aegypti
-
larva the protein localizes to the apical side of posterior midgut epithelial cells of larva Aedes aegypti
-
midgut the protein localizes to the apical side of posterior midgut epithelial cells of larva Aedes aegypti
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O
-
 Aedes aegypti L-Ala + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Aedes aegypti L-Leu + 4-nitroaniline
-
 ?
L-Val-4-nitroanilide + H2O
-
 Aedes aegypti L-Val + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
aminopeptidase N
-
 Aedes aegypti
APN1
-
 Aedes aegypti

General Information

General Information Comment Organism
physiological function aminopeptidase N is a putative receptor of Bacillus thuringiensis Cry11A toxin Aedes aegypti