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Literature summary for 3.4.11.2 extracted from

  • Bhosale, M.; Pande, S.; Kumar, A.; Kairamkonda, S.; Nandi, D.
    Characterization of two M17 family members in Escherichia coli, Peptidase A and Peptidase B (2010), Biochem. Biophys. Res. Commun., 395, 76-81.
    View publication on PubMed
Search for more literature for 3.4.11.2

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Escherichia coli
bestatin
-
 Escherichia coli
pepstatin
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 L-Leu-7-amido-4-methylcoumarin
-
 Escherichia coli
0.31
-
 Gly-7-amido-4-methylcoumarin
-
 Escherichia coli
0.34
-
 L-Met-7-amido-4-methylcoumarin
-
 Escherichia coli
0.382
-
 L-Lys-4-nitroanilide
-
 Escherichia coli
0.445
-
 L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatography columns like DEAE cellulose, Q-sepharose and butyl toyopearl, gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.83
-
 value about, substrate L-Asp-7-amido-4-methylcoumarin Escherichia coli
3.3
-
 value about, substrate L-Trp-7-amido-4-methylcoumarin Escherichia coli
11.7
-
 value about, substrate L-Gln-7-amido-4-methylcoumarin Escherichia coli
15
-
 value about, substrate L-Tyr-7-amido-4-methylcoumarin Escherichia coli
16.7
-
 value about, substrate L-Thr-7-amido-4-methylcoumarin Escherichia coli
33.3
-
 value about, substrate L-Pro-7-amido-4-methylcoumarin Escherichia coli
58.3
-
 value about, substrate L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin Escherichia coli
83.3
-
 value about, substrate L-Met-7-amido-4-methylcoumarin Escherichia coli
91.7
-
 value about, substrate Gly-7-amido-4-methylcoumarin Escherichia coli
116.7
-
 value about, substrate L-Leu-7-amido-4-methylcoumarin Escherichia coli
233.3
-
 value about, substrate L-Lys-4-nitroanilide Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O assay at pH 8.0, 37°C Escherichia coli ?
-
 ?
Gly-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + Gly
-
 ?
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Ala-L-Ala-L-Phe
-
 ?
L-Asp-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Asp
-
 ?
L-Gln-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Gln
-
 ?
L-Leu-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Leu
-
 ?
L-Lys-4-nitroanilide assay at pH 8.0, 37°C Escherichia coli 4-nitroaniline + L-Lys
-
 ?
L-Met-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Met
-
 ?
L-Pro-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Pro
-
 ?
L-Thr-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Thr
-
 ?
L-Trp-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Trp
-
 ?
L-Tyr-7-amido-4-methylcoumarin + H2O assay at pH 8.0, 37°C Escherichia coli 7-amino-4-methylcoumarin + L-Tyr
-
 ?
oxidized insulin B chain + H2O assay at pH 8.0, 37°C Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
PepN
-
 Escherichia coli
peptidase N
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 melting temperature Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
53
-
 L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin
-
 Escherichia coli
80
-
 L-Met-7-amido-4-methylcoumarin
-
 Escherichia coli
90
-
 L-Leu-7-amido-4-methylcoumarin
-
 Escherichia coli
92
-
 Gly-7-amido-4-methylcoumarin
-
 Escherichia coli
290
-
 L-Lys-4-nitroanilide
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
126
-
 L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin
-
 Escherichia coli
235
-
 L-Met-7-amido-4-methylcoumarin
-
 Escherichia coli
292
-
 Gly-7-amido-4-methylcoumarin
-
 Escherichia coli
330
-
 L-Leu-7-amido-4-methylcoumarin
-
 Escherichia coli
1000
-
 L-Lys-4-nitroanilide
-
 Escherichia coli