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Literature summary for 3.4.11.2 extracted from

  • Zhang, R.; Hua, G.; Andacht, T.M.; Adang, M.J.
    A 106-kDa aminopeptidase is a putative receptor for Bacillus thuringiensis Cry11Ba toxin in the mosquito Anopheles gambiae (2008), Biochemistry, 47, 11263-11272.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.2

Cloned(Commentary)

Cloned (Comment) Organism
a 70 kDa fragment of the 106 kDa APN is expressed in Escherichia coli BL21-CodonPlus (DE3)/pRIL cells Anopheles gambiae

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border membrane
-
 Anopheles gambiae 31526
-
membrane
-
 Anopheles gambiae 16020
-
microvillus microvilli of the posterior midgut Anopheles gambiae 5902
-
vesicle
-
 Anopheles gambiae 31982
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the protein has a zinc-binding motif (HEIAH) Anopheles gambiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
 gel filtration Anopheles gambiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Anopheles gambiae APN is a specific binding protein, and a putative midgut receptor, for Bacillus thuringiensis Cry11Ba toxin ?
-
 ?
additional information Anopheles gambiae CDC G3 APN is a specific binding protein, and a putative midgut receptor, for Bacillus thuringiensis Cry11Ba toxin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Anopheles gambiae C5H690
-
-
Anopheles gambiae CDC G3 C5H690
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Anopheles gambiae

Purification (Commentary)

Purification (Comment) Organism
HiTrap Ni2+-chelating HP column chromatography Anopheles gambiae

Source Tissue

Source Tissue Comment Organism Textmining
midgut
-
 Anopheles gambiae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-p-nitroanilide + H2O
-
 Anopheles gambiae L-Ala + p-nitroaniline
-
 ?
L-Ala-p-nitroanilide + H2O
-
 Anopheles gambiae CDC G3 L-Ala + p-nitroaniline
-
 ?
additional information APN is a specific binding protein, and a putative midgut receptor, for Bacillus thuringiensis Cry11Ba toxin Anopheles gambiae ?
-
 ?
additional information APN is a specific binding protein, and a putative midgut receptor, for Bacillus thuringiensis Cry11Ba toxin Anopheles gambiae CDC G3 ?
-
 ?

Synonyms

Synonyms Comment Organism
aminopeptidase N
-
 Anopheles gambiae
APN2
-
 Anopheles gambiae