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Literature summary for 3.4.11.2 extracted from

  • Shitomi, Y.; Hayakawa, T.; Hossain, D.M.; Higuchi, M.; Miyamoto, K.; Nakanishi, K.; Sato, R.; Hori, H.
    A novel 96-kDa aminopeptidase localized on epithelial cell membranes of Bombyx mori midgut, which binds to Cry1Ac toxin of Bacillus thuringiensis (2006), J. Biochem., 139, 223-233.
    View publication on PubMed
Search for more literature for 3.4.11.2

Activating Compound

Activating Compound Comment Organism Structure
1,10-phenanthroline 50% activation at 10 mM Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ 9% inhibition at 1 mM Bombyx mori
additional information no inhibition by EDTA Bombyx mori
Zn2+ 80% inhibition at 1 mM Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information reaction kinetics, kinetics of the interation between APN96 and insecticidal Cry1Ac toxin, pH 8.0 Bombyx mori
0.13
-
 L-Ala-4-nitroanilide pH 8.0 Bombyx mori

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border
-
 Bombyx mori 5903
-
membrane epithelial cell, brush border Bombyx mori 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information APN96 is not affected by Co2+, Cu2+, and Ca2+ Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96400
-
 2 * 96400, isozyme APN96, SDS-PAGE Bombyx mori
190000
-
 gel filtration Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
 isozyme APN96
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isozyme APN96 shows N-linked bi-antennal oligosaccharide or an O-linked mucin type one Bombyx mori

Purification (Commentary)

Purification (Comment) Organism
native APN96 from midgut brush border membranes to homogeneity by solubilization with Triton X-100, ion exchange chromatography, and 2 steps of gel filtration Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
epithelial cell
-
 Bombyx mori
-
larva
-
 Bombyx mori
-
midgut
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
144.5
-
 purified isozyme APN96, substrate L-Leu-4-nitroanilide Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-4-nitroanilide + H2O preferred substrate Bombyx mori L-Ala + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O low activity Bombyx mori L-Arg + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Bombyx mori L-Leu + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O
-
 Bombyx mori L-Lys + 4-nitroaniline
-
 ?
L-Met-4-nitroanilide + H2O
-
 Bombyx mori L-Met + 4-nitroaniline
-
 ?
L-Val-4-nitroanilide + H2O very low activity Bombyx mori L-Val + 4-nitroaniline
-
 ?
additional information substrate specificity, overview, no activity with L-Gly-4-nitroanilide and L-Pro-4-nitroanilide, the enzyme binds to Cry1Ac toxin of Bacillus thuringiensis, not with Cry1Aa or Cey1Ab toxins, the interaction is inhibited by GalNAc Bombyx mori ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 96400, isozyme APN96, SDS-PAGE Bombyx mori
More peptide mass fingerprinting, overview Bombyx mori

Synonyms

Synonyms Comment Organism
aminopeptidase N
-
 Bombyx mori
APN96
-
 Bombyx mori
More isozyme APN96 belongs to the APN3 family Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Bombyx mori