Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue | Novosphingobium capsulatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Novosphingobium capsulatum | |
additional information | no inhibition by EDTA and PMSF | Novosphingobium capsulatum | |
Zn2+ | slight inhibition, as ferrocyanide, iodate, and phosphate, Ki value depends on solubility of the salt | Novosphingobium capsulatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
L-Ala-2-naphthylamide | pH 7.5, 43°C | Novosphingobium capsulatum | |
14 | - |
L-Ala-4-nitroanilide | pH 7.5, 43°C | Novosphingobium capsulatum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Novosphingobium capsulatum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme contains the zinc binding motif HEXXH | Novosphingobium capsulatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Novosphingobium capsulatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Novosphingobium capsulatum | - |
- |
- |
Novosphingobium capsulatum IFO 12533 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from culture broth supernatant by ultrafiltration, ion exchange chromatography, and gel filtration | Novosphingobium capsulatum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105 | - |
purified enzyme | Novosphingobium capsulatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-4-nitroanilide + H2O | 14% of the activity with L-Ala-4-nitroanilide | Novosphingobium capsulatum | Gly + 4-nitroaniline | - |
? | |
L-Ala-2-naphthylamide + H2O | - |
Novosphingobium capsulatum | L-Ala + 2-naphthylamine | - |
? | |
L-Ala-4-nitroanilide + H2O | best substrate | Novosphingobium capsulatum | L-Ala + 4-nitroaniline | - |
? | |
L-Asp-4-nitroanilide + H2O | 14% of the activity with L-Ala-4-nitroanilide | Novosphingobium capsulatum | L-Asp + 4-nitroaniline | - |
? | |
L-Glu-4-nitroanilide + H2O | 12% of the activity with L-Ala-4-nitroanilide | Novosphingobium capsulatum | L-Glu + 4-nitroaniline | - |
? | |
L-Leu-4-nitroanilide + H2O | 27% of the activity with L-Ala-4-nitroanilide | Novosphingobium capsulatum | L-Leu + 4-nitroaniline | - |
? | |
L-Lys-4-nitroanilide + H2O | 3% of the activity with L-Ala-4-nitroanilide | Novosphingobium capsulatum | L-Lys + 4-nitroaniline | - |
? | |
L-Met-4-nitroanilide + H2O | 24% of the activity with L-Ala-4-nitroanilide | Novosphingobium capsulatum | L-Met + 4-nitroaniline | - |
? | |
additional information | no activity with 4-nitroanilides of Pro, Ile, Val, and Phe | Novosphingobium capsulatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Novosphingobium capsulatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Novosphingobium capsulatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
20 min, purified enzyme, loss of 40% activity | Novosphingobium capsulatum |
50 | - |
20 min, purified enzyme, inactivation | Novosphingobium capsulatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
860 | - |
L-Ala-2-naphthylamide | pH 7.5, 43°C | Novosphingobium capsulatum | |
7600 | - |
L-Ala-4-nitroanilide | pH 7.5, 43°C | Novosphingobium capsulatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.4 | - |
Novosphingobium capsulatum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Novosphingobium capsulatum |