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Literature summary for 3.4.11.18 extracted from
- Biochemical characterization of recombinant methionine aminopeptidases (MAPs) from Mycobacterium tuberculosis H37Rv (2012), Mol. Cell. Biochem., 365, 191-202.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C105S | about 50% loss of activiy | Mycobacterium tuberculosis |
| D142A | almost complete loss of activity | Mycobacterium tuberculosis |
| T94C | slight enhancement of activity | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| actinonin | 0.01 mM, 40% loss of activity; 0.01 mM, 70% loss of activity | Mycobacterium tuberculosis |  |
| bestatin | 0.01 mM, 25% loss of activity; 0.01 mM, 50% loss of activity | Mycobacterium tuberculosis | |
| methionine | feedback inhibition. 70% inhibition at 0.1 mM | Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.154 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 0.193 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 0.466 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 0.52 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | and Ni2+, best activators. Highest activity at 0.1 mM | Mycobacterium tuberculosis | |
| Co2+ | may substitute for Ni2+. Highest activity at 0.04 mM | Mycobacterium tuberculosis | |
| Mn2+ | may substitute for Co2+ or Ni2+, highest activity at 0.4 mM | Mycobacterium tuberculosis | |
| Ni2+ | and Co2+, best activators. Highest activity at 0.1 mM | Mycobacterium tuberculosis | |
| Ni2+ | best activator. Highest activity at 0.1 mM | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
| 37000 | - |
x * 37000, SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WK19 | - |
- |
| Mycobacterium tuberculosis | P9WK21 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WK19 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WK21 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Met 4-nitroanilide + H2O | - |
Mycobacterium tuberculosis | L-Met + 4-nitroaniline | - |
? | |
| L-Met 4-nitroanilide + H2O | - |
Mycobacterium tuberculosis H37Rv | L-Met + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
| ? | x * 37000, SDS-PAGE, recombinant protein | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MAP-2 | - |
Mycobacterium tuberculosis |
| MAP11 | - |
Mycobacterium tuberculosis |
| mAPA | - |
Mycobacterium tuberculosis |
| MapB | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mycobacterium tuberculosis |
| 55 | - |
- |
Mycobacterium tuberculosis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
no activity above | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 0.08 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 0.08 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 0.133 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Mycobacterium tuberculosis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 153 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 171 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 404 | - |
L-Met 4-nitroanilide | presence of Co2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
| 867 | - |
L-Met 4-nitroanilide | presence of Ni2+, pH 8.0, 22°C | Mycobacterium tuberculosis | |
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