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Literature summary for 3.4.11.18 extracted from

  • Zhang, X.; Chen, S.; Hu, Z.; Zhang, L.; Wang, H.
    Expression and characterization of two functional methionine aminopeptidases from Mycobacterium tuberculosis H37Rv (2009), Curr. Microbiol., 59, 520-525.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes Rv0734 and Rv2861c, expression of the His-tagged isozymes in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ slight inhibition Mycobacterium tuberculosis
Cu2+
-
Mycobacterium tuberculosis
Fe2+
-
Mycobacterium tuberculosis
Ni2+
-
Mycobacterium tuberculosis
Zn2+ slight inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
Met-Ala-Ser pH 7.5, 37°C, isozyme MetAP1a Mycobacterium tuberculosis
0.058
-
Met-Gly-Met-Met pH 7.5, 37°C, isozyme MetAP1c Mycobacterium tuberculosis
0.328
-
Met-Gly-Met-Met pH 7.5, 37°C, isozyme MetAP1a Mycobacterium tuberculosis
0.394
-
Met-Ala-Ser pH 7.5, 37°C, isozyme MetAP1c Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates best Mycobacterium tuberculosis
Mg2+ activates slightly Mycobacterium tuberculosis
Mn2+ activates slightly Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28812
-
x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry Mycobacterium tuberculosis
32516
-
x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
strains H37Ra and H37Rv, two isozymes MtMetAP1a and MtMetAP1c encoded by genes Rv0734 and Rv2861c
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozymes from Escherichia coli, the tag is cleaved off by thrombin Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Ala-Pro-Thr + H2O
-
Mycobacterium tuberculosis Met + Ala-Pro-Thr
-
?
Met-Ala-Ser + H2O
-
Mycobacterium tuberculosis Met + Ala-Ser
-
?
Met-Gly-Gly + H2O
-
Mycobacterium tuberculosis Met + Gly-Gly
-
?
Met-Gly-Met-Met + H2O
-
Mycobacterium tuberculosis Met + Gly-Met-Met
-
?
Met-Leu-Phe + H2O
-
Mycobacterium tuberculosis Met + Leu-Phe
-
?
Met-Phe-Gly + H2O
-
Mycobacterium tuberculosis Met + Phe-Gly
-
?
Met-Pro-Gly-Gly + H2O
-
Mycobacterium tuberculosis Met + Pro-Gly-Gly
-
?
additional information isozyme substrate specificity, overview Mycobacterium tuberculosis ?
-
?

Subunits

Subunits Comment Organism
? x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
MetAP
-
Mycobacterium tuberculosis
MetAP1a
-
Mycobacterium tuberculosis
MetAP1c
-
Mycobacterium tuberculosis
methionine aminopeptidase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
isozyme MetAP1c Mycobacterium tuberculosis
55
-
isozyme MetAP1a Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
10 min, inactivation of isozyme MetAP1c, while isozyme MetAP1a retains 50% activity Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.5
-
Met-Gly-Met-Met pH 7.5, 37°C, isozyme MetAP1a Mycobacterium tuberculosis
19.8
-
Met-Ala-Ser pH 7.5, 37°C, isozyme MetAP1a, with Co2+ Mycobacterium tuberculosis
39.4
-
Met-Gly-Met-Met pH 7.5, 37°C, isozyme MetAP1c Mycobacterium tuberculosis
74.3
-
Met-Ala-Ser pH 7.5, 37°C, isozyme MetAP1c, with Co2+ Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mycobacterium tuberculosis