Cloned (Comment) | Organism |
---|---|
genes Rv0734 and Rv2861c, expression of the His-tagged isozymes in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight inhibition | Mycobacterium tuberculosis | |
Cu2+ | - |
Mycobacterium tuberculosis | |
Fe2+ | - |
Mycobacterium tuberculosis | |
Ni2+ | - |
Mycobacterium tuberculosis | |
Zn2+ | slight inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
Met-Ala-Ser | pH 7.5, 37°C, isozyme MetAP1a | Mycobacterium tuberculosis | |
0.058 | - |
Met-Gly-Met-Met | pH 7.5, 37°C, isozyme MetAP1c | Mycobacterium tuberculosis | |
0.328 | - |
Met-Gly-Met-Met | pH 7.5, 37°C, isozyme MetAP1a | Mycobacterium tuberculosis | |
0.394 | - |
Met-Ala-Ser | pH 7.5, 37°C, isozyme MetAP1c | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates best | Mycobacterium tuberculosis | |
Mg2+ | activates slightly | Mycobacterium tuberculosis | |
Mn2+ | activates slightly | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28812 | - |
x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry | Mycobacterium tuberculosis |
32516 | - |
x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
strains H37Ra and H37Rv, two isozymes MtMetAP1a and MtMetAP1c encoded by genes Rv0734 and Rv2861c | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes from Escherichia coli, the tag is cleaved off by thrombin | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Ala-Pro-Thr + H2O | - |
Mycobacterium tuberculosis | Met + Ala-Pro-Thr | - |
? | |
Met-Ala-Ser + H2O | - |
Mycobacterium tuberculosis | Met + Ala-Ser | - |
? | |
Met-Gly-Gly + H2O | - |
Mycobacterium tuberculosis | Met + Gly-Gly | - |
? | |
Met-Gly-Met-Met + H2O | - |
Mycobacterium tuberculosis | Met + Gly-Met-Met | - |
? | |
Met-Leu-Phe + H2O | - |
Mycobacterium tuberculosis | Met + Leu-Phe | - |
? | |
Met-Phe-Gly + H2O | - |
Mycobacterium tuberculosis | Met + Phe-Gly | - |
? | |
Met-Pro-Gly-Gly + H2O | - |
Mycobacterium tuberculosis | Met + Pro-Gly-Gly | - |
? | |
additional information | isozyme substrate specificity, overview | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
MetAP | - |
Mycobacterium tuberculosis |
MetAP1a | - |
Mycobacterium tuberculosis |
MetAP1c | - |
Mycobacterium tuberculosis |
methionine aminopeptidase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
isozyme MetAP1c | Mycobacterium tuberculosis |
55 | - |
isozyme MetAP1a | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, inactivation of isozyme MetAP1c, while isozyme MetAP1a retains 50% activity | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.5 | - |
Met-Gly-Met-Met | pH 7.5, 37°C, isozyme MetAP1a | Mycobacterium tuberculosis | |
19.8 | - |
Met-Ala-Ser | pH 7.5, 37°C, isozyme MetAP1a, with Co2+ | Mycobacterium tuberculosis | |
39.4 | - |
Met-Gly-Met-Met | pH 7.5, 37°C, isozyme MetAP1c | Mycobacterium tuberculosis | |
74.3 | - |
Met-Ala-Ser | pH 7.5, 37°C, isozyme MetAP1c, with Co2+ | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |