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Literature summary for 3.4.11.18 extracted from

  • Yang, G.; Kirkpatrick, R.B.; Ho, T.; Zhang, G.F.; Liang, P.H.; Johanson, K.O.; Casper, D.J.; Doyle, M.L.; Marino, J.P., Jr.; Thompson, S.K.; Chen, W.; Tew, D.G.; Meek, T.D.
    Steady-State Kinetic Characterization of Substrates and Metal-Ion Specificities of the Full-Length and N-Terminally Truncated Recombinant Human Methionine Aminopeptidases (Type 2) (2001), Biochemistry, 40, 10645-10654.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
full-length and truncated forms of hMetAP2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the 107 N-terminal residues of MetAP2 are not required for either the peptidolytic activity of the enzyme or its stability. The N-terminally truncated enzyme and the full-length enzyme yield essentially identical kinetic behavior and physical properties Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
7-amino-4-methylcoumarin product inhibition Homo sapiens
Co2+ 1.0 mM CoCl2, 58% inhibition Homo sapiens
fumagillin
-
Homo sapiens
L-Met product inhibition Homo sapiens
L-Met-(RS)-sulfoxide
-
Homo sapiens
L-Nle product inhibition Homo sapiens
Mg2+ 1.0 mM MgCl2, 21% inhibition Homo sapiens
Mn2+ 0.1 mM MnCl2, 26% inhibition. 5.0 mM, 36% inhibition Homo sapiens
Zn2+ 0.1 mM, complete inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-values of truncated enzsme Homo sapiens
0.12
-
Met-7-amido-4-methylcoumarin pH 7.5, 30°C, in presence of 0.01 mM CoCl2 Homo sapiens
0.34
-
Met-Gly-Met-Met pH 7.5, 30°C Homo sapiens
0.38
-
Met-Ala-Ser pH 7.5, 30°C Homo sapiens
0.67
-
Met-4-nitroanilide pH 7.5, 30°C Homo sapiens
0.85
-
Met-Ser pH 7.5, 30°C Homo sapiens
1.2
-
Met-Val pH 7.5, 30°C Homo sapiens
1.3
-
Met-Thr pH 7.5, 30°C Homo sapiens
2
-
Nle-4-nitroanilide pH 7.5, 30°C Homo sapiens
2.8
-
Met-Pro pH 7.5, 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ at low concentrations, 0.01 mM, Co2+ plays a key role in the stimulation of enzyme activity. It is still questionable that Co(II) is the physically relevant divalent cation Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52790
-
MALDI-MS Homo sapiens
54700
-
analytical ultracentrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Met-peptide + H2O Homo sapiens the enzyme is directly involved in the regulation of growth factor-stimulated endothelial cell proliferation Met + peptide
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
methionine aminopeptidase type 2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Storage Stability

Storage Stability Organism
4°C, MetAP2 is stable for several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-4-nitroanilide + H2O
-
Homo sapiens Met + 4-nitroaniline
-
?
Met-7-amido-4-methylcoumarin + H2O
-
Homo sapiens Met + 7-amino-4-methylcoumarin
-
?
Met-Ala + H2O
-
Homo sapiens Met + Ala
-
?
Met-Ala-Ser + H2O
-
Homo sapiens Met + Ala-Ser
-
?
Met-Gly-Met-Met + H2O
-
Homo sapiens Gly-Met-Met + Met
-
?
Met-Gly-Met-Met + H2O
-
Homo sapiens Met + Gly-Met-Met
-
?
Met-peptide + H2O the enzyme is directly involved in the regulation of growth factor-stimulated endothelial cell proliferation Homo sapiens Met + peptide
-
?
Met-Pro + H2O
-
Homo sapiens Met + Pro
-
?
Met-Ser + H2O
-
Homo sapiens Met + Ser
-
?
Met-Thr + H2O
-
Homo sapiens Met + Thr
-
?
Met-Val + H2O
-
Homo sapiens Met + Val
-
?
additional information while the nature of the P1' residue of peptide substrates dictates the substrate specificity in the active site of metAP2 the P2' residue appears to play a key role in the kinetics of peptidolysis. The catalytic efficiency of dipeptide substrates is at least 250fold lower than those of the tripeptides. When the P1' amino acid of the peptide substrate is sterically large such as with L-Leu, MetAP2 exhibits no detectable activity. L-Nle is the only amino acid residue other than L-Met that can be accomodated at the N-terminus of a substrate Homo sapiens ?
-
?
Nle-4-nitroanilide + H2O
-
Homo sapiens Nle + 4-nitroaniline
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information turnover-numbers of truncated enzyme Homo sapiens
0.00617
-
Met-Ser pH 7.5, 30°C Homo sapiens
0.01
-
Met-Thr pH 7.5, 30°C Homo sapiens
0.0133
-
Met-Val pH 7.5, 30°C Homo sapiens
0.0633
-
Met-7-amido-4-methylcoumarin pH 7.5, 30°C, in presence of 0.01 mM CoCl2 Homo sapiens
0.0733
-
Nle-4-nitroanilide pH 7.5, 30°C Homo sapiens
0.13
-
Met-Pro pH 7.5, 30°C Homo sapiens
0.158
-
Met-4-nitroanilide pH 7.5, 30°C Homo sapiens
2.83
-
Met-Gly-Met-Met pH 7.5, 30°C Homo sapiens
3.5
-
Met-Ala-Ser pH 7.5, 30°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000016
-
fumagillin pH 7.5, 30°C Homo sapiens
0.02
-
L-Met pH 7.5, 30°C Homo sapiens
0.18
-
L-Met-(RS)-sulfoxide pH 7.5, 30°C Homo sapiens
0.29
-
7-amino-4-methylcoumarin pH 7.5, 30°C Homo sapiens