Any feedback?
Literature summary for 3.4.11.18 extracted from
- Purification and characterization of a methionine aminopeptidase from Saccharomyces cerevisiae (1990), J. Biol. Chem., 265, 19892-19897.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Saccharomyces cerevisiae |  |
| EDTA | - |
Saccharomyces cerevisiae | |
| Hg2+ | HgCl2 | Saccharomyces cerevisiae | |
| nitrilotriacetic acid | - |
Saccharomyces cerevisiae | |
| p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae | |
| Zn2+ | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
1 * 34000, SDS-PAGE | Saccharomyces cerevisiae |
| 36000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Met-Ala-Ser + H2O | also cleaves methionine from other tripeptides with a relatively small and/or uncharged penultimate amino acid residue (Pro, Gly, Val, Thr, or Ser) but not if bulky and/or charged (Arg, His, Leu, Met, or Tyr) | Saccharomyces cerevisiae | Met + Ala-Ser | - |
? | |
| additional information | no activity with methionine 4-nitroanilide | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Saccharomyces cerevisiae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 60% of activity maximum, 50°C: about 70% of activity maximum | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.8 | about 50% of activity maximum at pH 6 and 7.8 | Saccharomyces cerevisiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
8.8 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
