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Literature summary for 3.4.11.16 extracted from

  • Hooper, N.M.; Hesp, R.J.; Tieku, S.
    Metabolism of aspartame by human and pig intestinal microvillar peptidases (1994), Biochem. J., 298, 635-639.
No PubMed abstract available
Search for more literature for 3.4.11.16

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.11
-
 alpha-Asp-Phe
-
 Sus scrofa
4.96
-
 Aspartame
-
 Sus scrofa
7.48
-
 alpha-Asp-Phe-NH2
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Peptides + H2O Sus scrofa
-
 ?
-
 ir
Peptides + H2O Homo sapiens involved in intestinal breakdown and uptake of aspartame ?
-
 ir

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
duodenal mucosa
-
 Homo sapiens
-
duodenal mucosa
-
 Sus scrofa
-
ileal mucosa no activity Homo sapiens
-
intestine
-
 Homo sapiens
-
intestine
-
 Sus scrofa
-
jejunum
-
 Sus scrofa
-
jejunum 75% of activity of human duodenal mucosa Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13 16 substrate-dependent Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-Asp-Phe + H2O
-
 Homo sapiens Asp + Phe
-
 ir
alpha-Asp-Phe + H2O
-
 Sus scrofa Asp + Phe
-
 ir
alpha-Asp-Phe-Met + H2O
-
 Homo sapiens Asp + Phe-Met
-
 ir
alpha-Asp-Phe-Met + H2O
-
 Sus scrofa Asp + Phe-Met
-
 ir
alpha-Asp-Phe-NH2 + H2O
-
 Homo sapiens Asp + Phe-NH2
-
 ir
alpha-Asp-Phe-NH2 + H2O
-
 Sus scrofa Asp + Phe-NH2
-
 ir
aspartame + H2O
-
 Sus scrofa ?
-
 ?
Peptides + H2O
-
 Sus scrofa ?
-
 ir
Peptides + H2O involved in intestinal breakdown and uptake of aspartame Homo sapiens ?
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
117
-
 alpha-Asp-Phe
-
 Sus scrofa
230
-
 alpha-Asp-Phe-NH2
-
 Sus scrofa
238
-
 Aspartame
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens
7
-
 assay at Sus scrofa