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Literature summary for 3.4.11.15 extracted from

  • Yasuhara, T.; Nakai, T.; Ohashi, A.
    Aminopeptidase Y, a new aminopeptidase from Saccharomyces cerevisiae. Purification, properties, localization, and processing by protease B (1994), J. Biol. Chem., 269, 13644-13650.
    View publication on PubMed
Search for more literature for 3.4.11.15

Activating Compound

Activating Compound Comment Organism Structure
additional information activation of 75000 Da-proform to 70000 Da active form by protease B Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 95% inhibition at 1 mM Saccharomyces cerevisiae
amastatin 90% inhibition at 0.02 mM Saccharomyces cerevisiae
bestatin 48% inhibition at 0.02 mM Saccharomyces cerevisiae
Cu2+ 90% inhibition at 0.5 mM Saccharomyces cerevisiae
diphosphate 93% inhibition at 1 mM Saccharomyces cerevisiae
dithiothreitol 94% inhibition at 1 mM Saccharomyces cerevisiae
EDTA 99% inhibition at 1 mM Saccharomyces cerevisiae
EGTA 99.9% inhibition at 1 mM Saccharomyces cerevisiae
Mn2+ 67% inhibition at 0.5 mM Saccharomyces cerevisiae
p-chloromercuribenzoic acid 81% inhibition at 0.1 mM Saccharomyces cerevisiae
Triphosphate 99.9% inhibition at 1 mM Saccharomyces cerevisiae
Zn2+ 0.81-0.84 mol per mol enzyme; 72% inhibition at 0.5 mM Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
autophagic vacuole
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required Saccharomyces cerevisiae
Co2+ 800-900% activation by 0.5 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 1 * 70000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 2 molecular forms
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate-dependent Saccharomyces cerevisiae
0.028
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Arg)3 + H2O
-
 Saccharomyces cerevisiae Arg + Arg-Arg
-
 ir
(Leu)3 + H2O
-
 Saccharomyces cerevisiae Leu + Leu-Leu
-
 ir
(Lys)3 + H2O
-
 Saccharomyces cerevisiae Lys + Lys-Lys
-
 ir
Ala-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Ala + 7-amino-4-methylcoumarin
-
 ir
Arg-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Arg + 7-amino-4-methylcoumarin
-
 ir
Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Saccharomyces cerevisiae Arg + Arg-4-methylcoumaryl-7-amide
-
 ir
Arg-Arg-Lys-Ala-Leu-Ser-Pro + H2O
-
 Saccharomyces cerevisiae Arg + Arg-Lys-Ala-Leu-Ser-Pro
-
 ir
Arg-Gly-Tyr-Ser-Leu-Gly + H2O
-
 Saccharomyces cerevisiae Arg + Gly-Tyr-Ser-Leu-Gly
-
 ir
Arg-Val + H2O
-
 Saccharomyces cerevisiae Arg + Val
-
 ir
Arg-Val-OMe + H2O
-
 Saccharomyces cerevisiae Arg + Val-OMe
-
 ir
Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
-
 Saccharomyces cerevisiae Arg + Val-Tyr-Ile-His-Pro-Phe
-
 ir
Leu-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Leu + 7-amino-4-methylcoumarin
-
 ir
Leu-Arg-Arg-Ala-Ser-Leu-Gly + H2O
-
 Saccharomyces cerevisiae Leu + Arg-Arg-Ala-Ser-Leu-Gly
-
 ir
Leu-Leu + H2O
-
 Saccharomyces cerevisiae Leu + Leu
-
 ir
Leu-Leu-OMe + H2O
-
 Saccharomyces cerevisiae Leu + Leu-OMe
-
 ir
Lys-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Lys + 7-amino-4-methylcoumarin
-
 ir
Lys-Ala-4-methylcoumaryl-7-amide + H2O
-
 Saccharomyces cerevisiae Lys + Ala-4-methylcoumaryl-7-amide
-
 ir
Met-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Met + 7-amino-4-methylcoumarin
-
 ir
Met-Ala-Ser + H2O
-
 Saccharomyces cerevisiae Met + Ala-Ser
-
 ir
Met-Arg-Phe + H2O
-
 Saccharomyces cerevisiae Met + Arg-Phe
-
 ir
Met-Gly-Gly + H2O
-
 Saccharomyces cerevisiae Met + Gly-Gly
-
 ir
additional information generally higher activities with dipeptide-4-methylcoumaryl-7-amides than with amino-acid-4-methylcoumaryl-7-amides Saccharomyces cerevisiae ?
-
 ?
Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
 Saccharomyces cerevisiae Pro + Phe-Arg-4-methylcoumaryl-7-amide
-
 ir
Ser-7-amido-4-methylcoumarin + H2O
-
 Saccharomyces cerevisiae Ser + 7-amino-4-methylcoumarin
-
 ir
Tyr-Gly-Gly-Phe-Leu + H2O
-
 Saccharomyces cerevisiae Tyr + Gly-Gly-Phe-Leu
-
 ir

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Saccharomyces cerevisiae