Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.14 extracted from

  • Chen, S.H.; Cao, M.J.; Huang, J.Z.; Wu, G.P.
    Identification of a puromycin-sensitive aminopeptidase from zebrafish (Danio rerio) (2011), Comp. Biochem. Physiol. B, 159, 10-17.
    View publication on PubMed
Search for more literature for 3.4.11.14

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 19.4% residual activity at 0.2 mM Danio rerio
bestatin 1.1% residual activity at 0.05 mM Danio rerio
CdCl2 6.2% residual activity at 1 mM Danio rerio
CuSO4 0.6% residual activity at 1 mM Danio rerio
E-64 27.2% residual activity at 0.015 mM Danio rerio
EDTA 17.5% residual activity at 5 mM, 3,3% residual activity after extensively dialysis against 1 mM, restored by 0.01 mM Zn2+ to 57.8% activity Danio rerio
EGTA 24.3% residual activity at 5 mM Danio rerio
L-arginine 19.3% residual activity at 5 mM Danio rerio
L-cysteine non-competitive, 7.3% residual activity at 5 mM Danio rerio
L-isoleucine 20.1% residual activity at 5 mM Danio rerio
L-leucine 6.9% residual activity at 5 mM Danio rerio
L-phenylalanine 21.1% residual activity at 5 mM Danio rerio
L-tryptophan 15.5% residual activity at 5 mM Danio rerio
L-valine 23.7% residual activity at 5 mM Danio rerio
puromycin 9.5% residual activity at 0.01 mM Danio rerio
ZnSO4 24% residual activity at 1 mM Danio rerio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
0.0019
-
 L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
0.0033
-
 L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
0.0052
-
 L-lysine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
0.0066
-
 L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
0.01
-
 L-methionine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
0.035
-
 L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
0.045
-
 L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
0.24
-
 glycine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 gel filtration Danio rerio

Organism

Organism UniProt Comment Textmining
Danio rerio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange chromatography (DEAE), hydroxyapatite chromatography, ammonium sulfate precipitation, hydrophobic interaction chromatography Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine-4-methyl-coumaryl-7-amide + H2O
-
 Danio rerio glycine + 7-amino-4-methylcoumarin
-
 ?
L-alanine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-arginine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-arginine + 7-amino-4-methylcoumarin
-
 ?
L-leucine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-lysine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-lysine + 7-amino-4-methylcoumarin
-
 ?
L-methionine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-methionine + 7-amino-4-methylcoumarin
-
 ?
L-proline-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-proline + 7-amino-4-methylcoumarin
-
 ?
L-tyrosine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-tyrosine + 7-amino-4-methylcoumarin
-
 ?
L-valine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-valine + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
monomer 98000, SDS-PAGE, gel filtration Danio rerio

Synonyms

Synonyms Comment Organism
puromycin-sensitive aminopeptidase
-
 Danio rerio

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Danio rerio

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 45 unstable above 50°C Danio rerio

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
1.1
-
 L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.8
-
 L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
8.2
-
 L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
10.3
-
 glycine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
17.1
-
 L-lysine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
25.6
-
 L-methionine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
38.5
-
 L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
48.9
-
 L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Danio rerio

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 activity decreases rapidly when pH value higher or lower than 7.0 Danio rerio

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.27
-
 L-cysteine pH 7.0, 37°C Danio rerio

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
40
-
 glycine-4-methyl-coumaryl-7-amide pH 7.0, 37°C Danio rerio
170
-
 L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
260
-
 L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
870
-
 L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
1400
-
 L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
2500
-
 L-methionine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3000
-
 L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3300
-
 L-lysine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
4200
-
 L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio