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Literature summary for 3.4.11.14 extracted from

  • Yamamoto, Y.; Li, Y.H.; Huang, K.; Ohkubo, I.; Nishi, K.
    Isolation and characterization of an alanyl aminopeptidase from rat liver cytosol as a puromycin-sensitive enkephalin-degrading aminopeptidase (1998), Biol. Chem., 379, 711-719.
    View publication on PubMed
Search for more literature for 3.4.11.14

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 5 mM, 2fold stimulation Rattus norvegicus

General Stability

General Stability Organism
inactivated after only one cycle of freezing and thawing Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, complete inhibition Rattus norvegicus
actinonin 0.1 mM, complete inactivation Rattus norvegicus
amastatin 0.1 mM, complete inactivation Rattus norvegicus
antipain 0.1 mM, 39% inhibition Rattus norvegicus
arphamenine A 0.1 mM, 80% inactivation Rattus norvegicus
benzamidine 1 mM, 14% inhibition Rattus norvegicus
bestatin 0.1 mM complete inactivation Rattus norvegicus
Cd2+ 1 mM, complete inhibition Rattus norvegicus
chymostatin 0.1 mM, 83% inhibition Rattus norvegicus
Co2+ 1 mM, complete inhibition Rattus norvegicus
Cu2+ 1 mM, complete inhibition Rattus norvegicus
EDTA 2 mM, 26% inhibition Rattus norvegicus
iodoacetamide 1 mM, 57% inhibition Rattus norvegicus
iodoacetic acid 1 mM, 11% inhibition Rattus norvegicus
leuhistin 0.1 mM, complete inactivation Rattus norvegicus
leupeptin 0.1 mM, 19% inhibition Rattus norvegicus
Mg2+ 1 mM, 20% inhibition Rattus norvegicus
Mn2+ 1 mM, 69% inhibition Rattus norvegicus
NEM 1 mM, 43% inhibition Rattus norvegicus
Ni2+ 1 mM, 90% inhibition Rattus norvegicus
phosphoramidon 0.1 mM, 17% inhibition Rattus norvegicus
PMSF 1 mM, 40% inhibition; 1 mM, 64% inhibition Rattus norvegicus
puromycin 0.1 mM, complete inactivation, IC50: 0.0006 mM Rattus norvegicus
Zn2+ 1 mM, complete inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.03
-
 Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.11
-
 Lys-Ala-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.115
-
 Met-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.115
-
 Lys-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.17
-
 Tyr-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.22
-
 Leu-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.235
-
 Ala-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.26
-
 Phe-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.5
-
 Pro-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 1 * 90000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus
100000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, pH 7.5, 20 mM Tris-buffered saline, stable for several weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-7-amido-4-methylcoumarin
-
 Rattus norvegicus Ala + 7-amino-4-methylcoumarin
-
 ?
Arg-7-amido-4-methylcoumarin 36% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Arg + 7-amino-4-methylcoumarin
-
 ?
Arg-Arg-7-amido-4-methylcoumarin 13% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus ?
-
 ?
Leu-7-amido-4-methylcoumarin 90% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Leu + 7-amino-4-methylcoumarin
-
 ?
Lys-7-amido-4-methylcoumarin 93% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Lys + 7-amino-4-methylcoumarin
-
 ?
Lys-Ala-7-amido-4-methylcoumarin 79% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus ?
-
 ?
Met-7-amido-4-methylcoumarin 97% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Met + 7-amino-4-methylcoumarin
-
 ?
additional information no activity with: Gly-Pro-7-amido-4-methylcoumarin, butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, butyloxycarbonyl-Val–Pro-Arg-7-amido-4-methylcoumarin,succinyl-Ala-Ala-Ala-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, succinyl-Gly-Pro-7-amido-4-methylcoumarin, succinyl-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin, succinyl-Ile-Ile-Trp-7-amido-4-methylcoumarin, benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin Rattus norvegicus ?
-
 ?
Phe-7-amido-4-methylcoumarin 84% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Phe + 7-amino-4-methylcoumarin
-
 ?
Pro-7-amido-4-methylcoumarin 39% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Pro + 7-amino-4-methylcoumarin
-
 ?
Pro-Phe-Arg-7-amido-4-methylcoumarin 20% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus ?
-
 ?
Tyr-7-amido-4-methylcoumarin + H2O 99% of the activity with Ala-7-amido-4-methylcoumarin Rattus norvegicus Tyr + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 90000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, stable up to Rattus norvegicus
50
-
 30 min, 50% loss of activity Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 Arg-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.13
-
 Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
0.97
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
1.5
-
 Pro-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
4.5
-
 Lys-Ala-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
7.7
-
 Tyr-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
11.3
-
 Leu-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
12.8
-
 Phe-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
13.1
-
 Ala-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
14.3
-
 Met-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus
19.5
-
 Lys-7-amido-4-methylcoumarin pH 7.5, 37°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 55% of maximal activity, pH 8.5: about 50% of maximal activity, hydrolysis of Ala-7-amido-4-methylcoumarin Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9.5 4°C, 24 h, stable Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus chromatofocusing 4.7 4.6

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0006
-
 0.1 mM, complete inactivation, IC50: 0.0006 mM Rattus norvegicus puromycin