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Literature summary for 3.4.11.13 extracted from

  • Kessler, E.; Yaron, A.
    Extracellular aminopeptidase from Clostridium histolyticum (1976), Methods Enzymol., 45, 544-552.
    View publication on PubMed

General Stability

General Stability Organism
very sensitive to traces of Zn2+ Hathewaya histolytica

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Hathewaya histolytica
p-mercuribenzoate
-
Hathewaya histolytica
Zn2+
-
Hathewaya histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.45
-
Leu-Gly-Gly
-
Hathewaya histolytica
8.4
-
Phe-Gly-Gly
-
Hathewaya histolytica
18.5
-
Val-Gly-Gly
-
Hathewaya histolytica
20
-
Pro-Gly-Pro
-
Hathewaya histolytica
20
-
Pro-Gly-Pro-Pro
-
Hathewaya histolytica
40
-
Pro-Gly-Gly
-
Hathewaya histolytica
80
-
Ala-Gly-Gly
-
Hathewaya histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Hathewaya histolytica
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+
-
Hathewaya histolytica
Co2+ optiumum concentration 0.025 mM Hathewaya histolytica
Mn2+ best activator, optimum concentration 0.05 mM Hathewaya histolytica
Ni2+
-
Hathewaya histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
340000
-
gel filtration Hathewaya histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Peptides + H2O Hathewaya histolytica
-
?
-
?

Organism

Organism UniProt Comment Textmining
Hathewaya histolytica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hathewaya histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.5
-
-
Hathewaya histolytica

Storage Stability

Storage Stability Organism
-20°C, 0.05 M phosphate buffer or 0.05 M Na-acetate buffer, pH 5.6, 1 mM EDTA, several years, little loss of activity, can be restored by treatment with 2.5 mM dithiothreitol Hathewaya histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Ala)3 + H2O
-
Hathewaya histolytica Ala + Ala-Ala
-
ir
(Ala)4 + H2O
-
Hathewaya histolytica Ala + (Ala)3 or Ala-Ala + Ala-Ala ir
(Ala)4-NH2 + H2O
-
Hathewaya histolytica Ala + (Ala)3-NH2 or Ala-Ala + Ala-Ala-NH2 or (Ala)3 + Ala-NH2 ir
(Pro-Gly-Pro)4-6 + H2O
-
Hathewaya histolytica Pro + Gly-Pro-(Pro-Gly-Pro)3-5
-
ir
(Pro-Gly-Pro)4-6-OMe + H2O
-
Hathewaya histolytica Pro + Gly-Pro-(Pro-Gly-Pro)3-5-OMe
-
ir
Ala-Ala-Phe-Ala + H2O
-
Hathewaya histolytica Ala + Ala-Phe-Ala or Ala-Ala + Phe-Ala ir
Ala-Gly-Gly + H2O
-
Hathewaya histolytica Ala + Gly-Gly
-
ir
Ala-Lys + H2O
-
Hathewaya histolytica Ala + Lys
-
ir
Gly-Gly-Phe + H2O
-
Hathewaya histolytica Gly + Gly-Phe
-
ir
Gly-Gly-Phe-Ala + H2O
-
Hathewaya histolytica Gly + Gly-Phe-Ala or Gly-Gly + Phe-Ala ir
Gly-Phe-Ala + H2O
-
Hathewaya histolytica Gly + Phe-Ala
-
ir
Hyp-Ala-NH2 + H2O
-
Hathewaya histolytica Hyp-Ala + NH3
-
ir
Leu-Gly-Gly + H2O
-
Hathewaya histolytica Leu + Gly-Gly
-
ir
Met-Gly-Gly + H2O
-
Hathewaya histolytica Met + Gly-Gly
-
ir
Met-Met + H2O
-
Hathewaya histolytica Met + Met
-
ir
Peptides + H2O
-
Hathewaya histolytica ?
-
?
Phe-Gly-Gly + H2O
-
Hathewaya histolytica Phe + Gly-Gly
-
ir
Pro-Gly-Gly + H2O
-
Hathewaya histolytica Pro + Gly-Gly
-
ir
Pro-Gly-Pro + H2O
-
Hathewaya histolytica Pro + Gly-Pro
-
?
Pro-Gly-Pro-Pro + H2O
-
Hathewaya histolytica Pro + Gly-Pro-Pro
-
ir
Pro-Leu + H2O
-
Hathewaya histolytica Pro + Leu
-
ir
Pro-Phe + H2O
-
Hathewaya histolytica Pro + Phe
-
ir
Ser-Gly-Gly + H2O
-
Hathewaya histolytica Ser + Gly-Gly
-
ir
Val-Gly-Gly + H2O
-
Hathewaya histolytica Val + Gly-Gly
-
ir

Subunits

Subunits Comment Organism
hexamer 6 * 51000-59000, SDS-PAGE Hathewaya histolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Hathewaya histolytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
276
-
Val-Gly-Gly
-
Hathewaya histolytica
337
-
Pro-Gly-Pro-Pro
-
Hathewaya histolytica
561
-
Pro-Gly-Pro
-
Hathewaya histolytica
667
-
Phe-Gly-Gly
-
Hathewaya histolytica
917
-
Leu-Gly-Gly
-
Hathewaya histolytica
1360
-
Pro-Gly-Gly
-
Hathewaya histolytica
1830
-
Ala-Gly-Gly
-
Hathewaya histolytica